温度逆境锻炼对低温胁迫下番茄幼苗细胞膜脂质过氧化产物及抗氧化酶活性的影响  

Effects of Temperature Stress Acclimation on Peroxidation Product of Membranein Lipids and Anti-oxidative Enzyme Activities of Tomato Seedling under Cold Stress

作　　者：姜述君[1] 常缨[2] 范文艳[1] 黎玉梅[1] 金丽娜[1] 杨云强[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《中国农学通报》2007年第10期444-448,共5页Chinese Agricultural Science Bulletin

基　　金：黑龙江省教育厅资助项目"番茄温度逆境交叉适应机理的研究"(10513035)。

摘　　要：为了探索番茄植物交叉适应现象及其生理机制,以番茄品种东农704为材料,研究了低温和高温锻炼后番茄幼苗在2℃低温下叶片中丙二醛(MDA)和抗氧化酶活性的变化。结果显示,番茄幼苗经过适宜的低温和高温锻炼均可提高幼苗的抗冷性,并且高温锻炼在提高番茄幼苗抗冷性方面明显优于低温锻炼。2℃低温胁迫1d,未经锻炼幼苗与锻炼幼苗相比,其MDA含量明显升高,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性大幅度下降,即使在恢复生长2d后,MDA仍维持在较高的水平,SOD、POD和CAT活性水平也较低。番茄幼苗在低温和高温锻炼后、低温胁迫后和恢复2d后,MDA含量和酶保护系统变化各有异同。上述研究结果表明番茄植物具温度逆境交叉适应性,低温和高温锻炼诱导的番茄抗寒机制存在着差异性。To explore the characteristic and mechanism of cross adaptation of tomato to different temperature stress, the tomato variety （Dongnong 704） was used to research the changes of anti-oxidative enzyme activity and malondialdehyde （MDA） content of cold acclimation and heat acclimation seedling under 2℃ stress. Result showed that Optimum low temperature and high temperature acclimation could enhance the chilling resistance of tomato seedling at the chilling stress, and chilling resistance induced by high temper- ature acclimation was more than that induced by low temperature acclimation. The content of MDA the seedlings of non-acclimation was higher than in the acclimation seedling, and the activities of hyperoxide mutase （SOD）, peroxidase （POD） and catalase （CAT） significantly decreased under 2℃ low temperature stress for 1 day. The content of MDA was still higher and the activities of three anti-oxidative enzymes were lower after the recovery for 2 days. The changes of MDA and activities of three anti-oxidative enzymes of acclimation tomato seedlings showed a significant difference during cold acclimation, heat accli- mation, chilling stress and recovery growth. These results indicated that tomato possess a characteristic of cross adaptation to temperature adverse circumstance, and the mechanisms of chilling resistance induced by cold and heat acclimation were different.

关 键 词：番茄 温度逆境锻炼 低温胁迫 交叉适应 抗氧化酶活性 

分 类 号：S651[农业科学—果树学]