磁性细菌的电磁诱导分离及人工培养  

The electromagnetic inducing separation and artificial development of magnetic bacteria

作　　者：解宇[1] 汪浩[1] 

机构地区：[1]杭州师范大学基础医学部,浙江杭州310036

出　　处：《杭州师范学院学报（医学版）》2007年第4期217-221,共5页Journal of Hangzhou Teachers College :Medical Edition

基　　金：浙江省自然科学基金(Y205562);杭州师范大学科研基金(2005XYZ02)

摘　　要：目的探讨实验室内进行自然标本中磁性细菌的分离和人工培养。方法室外采取淤泥样本,以自行设计的电磁诱导法,在实验室内分离淤泥标本中的磁性细菌菌株。对分离的菌株,以松永氏等提案的培养液组方,并调节其中的营养成分、及培养环境的pH、DO、Cos,观察磁性细菌在人工培养液中的生长状况。结果所分离的磁性细菌,培养时受多种因素的影响,与一般微生物的培养相比,其特殊性表现为对培养液中的Fe3+浓度及培养液的溶解氧浓度较为敏感。所分离的磁性细菌菌株在实验室培养4 d,1 000 mL培养液中分离出17.3 g磁性细菌和3.2 mg磁珠。结论自然标本中磁性细菌菌株的分离和培养可在实验室内常规条件下进行。Objective To inquiry into a method for separating the magnetic bacteria from natural specimen and the artificial development of the magnetic bacteria in the laboratory. Methods The silt sample was collected from outdoors, the strain of magnetic bacteria within specimen of the silt was separated by a method of the electromagnetism induce developed by ourselves in the laboratory. The separated strain of magnetic bacteria were cultured in the nutrient fluid proposed by Matsunaga through regulating various nourishment composition and pH, DO, Cos of the nutrient fluid, and the growth of the magnetic bacteria in the artificial nutrient fluid was observed. Results The growth of the separated magnetic bacteria was affected by various factors. Compared with the general bacterial development, the separated magnetic bacteria were sensitive to the deliquescence oxygen and Fe^3＋ concentration in the nutrient fluid. After the separated magnetic bacteria had been cultured in the laboratory for 4 days, 17.3 g of the magnetic bacteria and 3.2mg of bacterial magnetic particles were separated from 1000ml of a the nutrient fluid. Conclusion Separation of the magnetic bacteria from natural specimen and the artificial development of the magnetic bacteria can be carried on in general laboratory.

关 键 词：磁性细菌 电磁诱导分离 人工培养 

分 类 号：R318[医药卫生—生物医学工程]