经颈动脉途径移植神经干细胞治疗缺血性脑卒中  

Treatment of stroke in rats by intracarotid transplantation of neural stem cells

作　　者：戴炯[1] 李善泉[1] 文立[1] 马政文[2] 富赛里[2] 智玲梅[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,200001 [2]上海交通大学医学院神经生物学实验室

出　　处：《中华实验外科杂志》2007年第11期1313-1316,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察经颈动脉途径移植神经干细胞(NSCs)治疗大鼠脑缺血再灌注模型的效果以及移植NSCs在大鼠脑内的状况。方法采用线栓法制作大鼠的大脑中动脉闭塞/再灌注(MCAO/R)模型;将大鼠随机分为MCAO/R+NSCs移植组、MCAO/R+培养液(DFNG)注射组和正常大鼠+NSCs移植组,MCAO/R+NSCs移植组和MCAO/R+DFNG注射组于建模后24 h经同侧颈内动脉分别注射NSCs和DFNG;对3组大鼠进行神经功能评估和病理组织学检查。结果整个观察过程中,正常大鼠+NSCs移植组大鼠未出现神经功能障碍,MCAO/R+NSCs移植组和MCAO/R +DFNG注射组大鼠手术建模后均出现明显的神经功能障碍,随后神经功能状况逐渐改善,在移植早期两组大鼠的神经功能评分差异无统计学意义(P>0.05),在移植后期(手术建模后10和14 d) MCAO/R+NSCs移植组大鼠的神经功能状况要优于MCAO/R+DFNG注射组(P<0.05,P<0.05);苏木素-伊红(HE)染色显示,MCAO/R+NSCs移植组和MCAO/R+DFNG注射组可见在右侧大脑中动脉供应的额顶叶和尾壳核区域缺血性损伤改变,而正常大鼠+NSCs移植组的脑组织结构正常;免疫荧光染色发现,MCAO/R+NSCs移植组可见有移植的NSCs存活、分布于缺血损伤的额顶叶和尾壳核区域,并有部分移植的NSCs开始朝着神经元方向分化,个别移植的NSCs则开始朝着星形胶质细胞方向分化;而正常大鼠+NSCs移植组和MCAO/R+DFNG注射组的大鼠中均未发现有这些现象。结论经颈动脉途径移植NSCs治疗缺血性脑卒中,可见有移植的NSCs存活、分布于缺血损伤区域,并朝神经元和星形胶质细胞方向分化来进行神经修复,从而改善神经功能障碍。Objective To observe the effect of intracarotid transplantation of neural stem ceils （NSCs） to treat cerebral ischemia/reperfusion model in rats and the status of transplanted NSCs in the ischemic brain tissue of these rats. Methods The middle cerebral artery occlusiovL/reperfusion （ MCAO/ R） models in SD rats were established with suture-occluded method. All rats were randomly divided into MCAO/R ＋ NSCs transplantation group, MCAO/R ＋ culturing solution （DFNG） administration group and normal rat ＋ NSCs transplantation group. Twenty-four h after ischemia, the rats of MCAO/R model were treated with ipsilateral intracarotid arterial injection of NSCs （MCAO/R ＋ NSCs transplantation group） or DFNG （MCAO/R ＋ DFNG administration group）. All rats of three groups were evaluated with the exami- nation of neurological function and pathological assessment. Results The neurological function of normal rat ＋ NSCs transplantation group was good during the experiment. The rats of MCAO/R ＋ NSCs transplanta- tion group and MCAO/R ＋ DFNG administration group showed typical presentations after MCAO/R. Their neurological function was improved gradually during 14 days postoperatively. No significant differences in neurological severity points during the early period after transplantation were found between the two groups. During the later period after transplantation （ 10 and 14 days after MCAO/R ） , the neurological function of MCAO/R ＋ NSCs transplantation group was better than MCAO/R ＋ DFNG administration group （Pl0d = 2.40 × 10^-5 ,Pl4d = 1.45 × 10^-5）. In the MCAO/R ＋ NSCs transplantation group and MCAO/R ＋ DFNG administration group, HE staining found the obvous ischemic damage in the right territory of frontoparietal lobe, caudate nucleus and putamen. The brain tissue was normal in the normal rat ＋ NSCs transplantation group. In the MCAO/R ＋ NSCs transplantation group, immunofluorescence staining showed that the transplanted NSCs were alive and distributed in the ischemic

关 键 词：卒中 神经干细胞 移植 

分 类 号：R651.12[医药卫生—外科学]