人胰岛素样生长因子-1基因表达载体的构建  

Construction of human insulin-like growth factor 1 gene expression vector

作　　者：吴建平[1] 蒲小勇[2] 周云峰[1] 王行环[2] 吴一龙[2] 韩雪[3] 王怀鹏[2] 胡礼泉[4] 叶林柏[3] 徐战平[2] 陈浩阳[2] 

机构地区：[1]武汉大学中南医院放化疗科,430070 [2]广东省人民医院泌尿外科 [3]武汉大学生命科学学院国家重点实验室 [4]武汉大学中南医院泌尿外科男科学研究中心

出　　处：《中华实验外科杂志》2007年第11期1396-1397,共2页Chinese Journal of Experimental Surgery

摘　　要：目的构建人胰岛素样生长因子-1(hIGF-1)基因的表达载体。方法通过聚合酶链反应(PCR)方法从PCDB-MGF-1质粒中扩增出392 bp的靶片段,将其黏端克隆至pET-His表达质粒,然后应用限制性酶切、PCR扩增及序列测定等技术进行鉴定。结果成功构建了hIGF-1表达片段,酶切及测序证实读码框正确。结论成功克隆hIGF-1表达载体,为体外表达蛋白和行免疫治疗勃起功能障碍奠定基础。Objective To construct expression plasmid of human insulin-growth factor-1. Methods Human insulin-like growth factor 1 （hIGF-1） gene fragment including 392 bp was obtained by PCR amplification from the PCDB-hIGF1 plasmid,cloned and identified by PCR, restrictive enzyme and sequencing. Results Haman insulin-like growth factor 1 expression vector was successfully cloned with correct open reading fragment. Conclusion hIGF-1 fragment was cloned into the pET-His expression vector.

关 键 词：人胰岛素样生长因子-1 克隆 

分 类 号：R341[医药卫生—基础医学] R698.2