大鼠异位节段小肠移植模型的建立  

Experience of segmental heterotopic small bowel transplantation model in rats

作　　者：游燊[1] 何晓顺[1] 熊俊[1] 郭志勇[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州510080

出　　处：《中华实验外科杂志》2007年第11期1430-1432,共3页Chinese Journal of Experimental Surgery

摘　　要：目的建立大鼠异位节段小肠移植模型。方法对100只雄性SD大鼠施行50次异位节段小肠移植,采用肠系膜上动脉-腹主动脉端侧吻合以及门静脉-左肾静脉套管吻合重建供肠血管,远端肠管腹壁造瘘。结果预实验阶段移植25只,存活3只,成功率12%;正式实验阶段25次手术,成功21次,成功率84%;供体手术时间(60±5)min,移植肠修整时间(15±5)min,受体手术时间(100±10)min。结论大鼠小肠移植中注重手术操作中的细节问题是建立稳定模型的关键。Objective To summarize the experience in surgical complication and raise the suecessful rate of segmental heterotopic small bowel transplantation （SBT） model in rats. Methods Fifty pairs of male SD rats were used as donors and recipients. The segmental small bowel with a vascular pedicle composed of superior mesenteric artery （SMA） and portal vein （PV） was harvested as the graft. Revascularization was accomplished by end-to-side anastomosis between donor SMA and recipient infrarenal aorta and cuffed end-to-end anasmosis between donor PV and left renal vein of recipient. The distal end of graft bowel formed an enterostoma. Results The sargical saccess rate was improved from 12% to 84% through 25 pairs in the first stage and 25 pairs in the second stage. Average operative time in donors was （60 ± 5） rain and （ 100 ＋ 10） min in recipients respectively,and the graft repair took （ 15＋5） min. Conclusion Paying more attention to some details of the surgical procedure is the key of establishing a stable model of small bowel transplantation in rats

关 键 词：小肠移植 并发症 模型 动物 

分 类 号：R656.7[医药卫生—外科学]