高灵敏度人血清降钙素放射免疫分析法的建立及其临床应用  

The Setting up of Human Serum Calcitonin Radioimmunoassay and Its Clinical Application

作　　者：詹志伟[1] 孟迅吾[1] 陈莉[1] 周学瀛[1] 刘书勤[1] 孔彦平[1] 胡莹莹[1] 

机构地区：[1]中国医学科学院中国协和医科大学协和医院,北京100730

出　　处：《中国医学科学院学报》1997年第3期236-240,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金!39370747

摘　　要：This study deals with the setting up of human serurn calcitonin radioimmunoassay (hCT-RIA) using the high titer and specific antibody which was prepared by our own laboratory. Thefinal dilutfon of the antibedy titer was 1: 1 200 000 when the binding rate of Bo/T was 3o%.The anthay’s affinity constant (Kd) was 2. 25 X 1O11L/M. No cross reaction was found be-tween the antithey and the folfowing eight different peptide hormones and proteins, viz.: TSH,T3, T4, ACTH, PTH, BGP, Insulin and Gastrin. The coefficients variation of intra and interassay were 3. 2% and 9. 0%, respectively. The mean recovery rate of CT was 99. 8%. The sen-sitivity bf this assay was 9. 4 pg/ml. The examination of this methodology showed that an its in-dices met the demands of RIA. There was no difference in the mean value of serum CT betweenthe two sexs in 232 normal subjects, but the serum CT concentration in those aged of sixty andover of men and women, were much lower than those aged of fourty group. Mean CT concentra-tbo was low in the patients with primary osteoporosis, but were significantly higher in those suffering from medullary thyroid carcinoma, primary hyperparathyroidism crisis and chronic renalfailure.This study deals with the setting up of human serurn calcitonin radioimmunoassay (hCT-RIA) using the high titer and specific antibody which was prepared by our own laboratory. Thefinal dilutfon of the antibedy titer was 1: 1 200 000 when the binding rate of Bo/T was 3o%.The anthay's affinity constant (Kd) was 2. 25 X 1O11L/M. No cross reaction was found be-tween the antithey and the folfowing eight different peptide hormones and proteins, viz.: TSH,T3, T4, ACTH, PTH, BGP, Insulin and Gastrin. The coefficients variation of intra and interassay were 3. 2% and 9. 0%, respectively. The mean recovery rate of CT was 99. 8%. The sen-sitivity bf this assay was 9. 4 pg/ml. The examination of this methodology showed that an its in-dices met the demands of RIA. There was no difference in the mean value of serum CT betweenthe two sexs in 232 normal subjects, but the serum CT concentration in those aged of sixty andover of men and women, were much lower than those aged of fourty group. Mean CT concentra-tbo was low in the patients with primary osteoporosis, but were significantly higher in those suffering from medullary thyroid carcinoma, primary hyperparathyroidism crisis and chronic renalfailure.

关 键 词：降钙素 人降钙素 血清 抗体 放射免疫测定 

分 类 号：R392－33[医药卫生—免疫学] R446.6[医药卫生—基础医学]