烟草黑胫病拮抗放线菌的分离与筛选  

Isolation and Screening of Antagonistic Actinomyces against Phytophthora parasitica var. nicotianae Tucker

作　　者：张永春[1] 黄镇[2] 杜怀敏[1] 陆宁[1] 谢已书[1] 王彬[3] 关国经[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳550003 [2]中国科学技术大学烟草与健康研究中心,合肥230052 [3]山东中烟工业公司济南卷烟厂,济南250100

出　　处：《中国烟草科学》2007年第5期5-8,共4页Chinese Tobacco Science

基　　金：贵州省烟草专卖局资助(2004-12)

摘　　要：从堆沤过圈肥的土壤中采集样品,用平板法对所采样品进行菌株分离、纯化培养,共获得原始放线菌101株,再通过室内拮抗试验,从随机28株试验菌株中初步获得16株具有一定拮抗作用的菌株。对拮抗效果较好的6株菌株以及抑菌圈较小、但其菌落较大的A15菌株进一步做盆栽防治试验。结果表明,A6的防治效果最好,达到55.26%,其它菌株效果一般。101 strains of original Actinomyces spp. were isolated from soils composted with barnyard manure, and 16 strains with definite antagonistic effect were selected from 28 random strains by laboratory antagonistic test. The results of pot-culture control test using 6 strains with good antagonistic effect and A15 strains With less inhibition zone and greater colony showed that prevention effect of A6 strain was the best, up to 55.26%, and that of the other strains was less.

关 键 词：烟草黑胫病 放线菌 拮抗 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]