人类白细胞Ⅱ类抗原DR、DQ基因型与妊娠期糖尿病的相关性研究  

Study on correlation between human leukocyte antigen class Ⅱ DR and DQ genotype and gestational diabetes mellitus.

作　　者：周信芳[1] 狄文英 陶建英[1] 蒋创[1] 戴建荣[1] 

机构地区：[1]苏州市立医院母子医疗保健中心妇产科,215002 [2]苏州红十字中心血站HLA实验室,215002

出　　处：《中国优生与遗传杂志》2007年第11期64-65,66,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨人类白细胞II类抗原DR、DQ基因型与妊娠期糖尿病的相关性。方法对26例GDM孕妇及同期入院的42例正常健康孕妇,采用序列特异性引物聚合酶链反应技术(PCR-SSP)检测HLA-II类抗原DR和DQ的等位基因。结果研究中发现,DQA1*0101、DQA1*0201、DQB1*0609、DRB l*07-DQA1*0201-DQB1*0201基因频率在GDM中显著高于正常对照组,两组比较,统计学有差异(Ρ<0.05)。DQB1*0301基因频率在GDM中显著低于正常对照组,两组比较,有统计学差异(Ρ<0.05)。结论人类白细胞II类抗原DR、DQ基因型与GDM的易感性和保护性存在关联。DQA1*0101、DQA1*0201、DQB1*0609、DRB l*07-DQA1*0201-DQB1*0201基因是GDM的易感基因。DQB1*0301基因是GDM的保护基因。Objective To investigate correlation between part of human leukocyte antigen （HLA） class Ⅱ DR and DQ genotype and gestational diabetes meUitus （GDM）. Methods ： 26 GDM women were served as study group, and 42 normal pregnant wonmen were selected as control group. DR and DQ of （HLA） class Ⅱ gene was examined by polymerase chain reaction sequence specific primer （PCR -SSP）. Results： The frequencies of DQA1 ＊ 0101, DQA1 ＊ 0201, DQB1 ＊ 0609, DRB1 ＊ 07 - DQA1 ＊ 0201 - DQB1 ＊ 0201 in study group were significantly increased compared with control group （ P 〈0. 05）, The frequencies of DQB1 ＊ 0301 in study group were significantly decline compared with control group （ P 〈 O. 05）. Conclusion ： The results suggest that there is correlation between DR and DQ of （HLA） class Ⅱ gene and gestational diabetes mellitus （GDM）. DQA1 ＊ 0101, DQA1 ＊ 0201, DQB1 ＊ 0609 and DRB1 ＊ 07 - DQA1 ＊ 0201 - DQB1 ＊ 0201 are the susceptible alleles of GDM. DQB1 ＊ 0301 is the protecting alleles of GDM.

关 键 词：人类白细胞Ⅱ类抗原 DR、DQ基因型 妊娠期糖尿病 易感基因 保护基因 

分 类 号：R714.25[医药卫生—妇产科学]