检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:杨绍兰[1] 陈妙金[1] 张波[1] 李鲜[1] 徐昌杰[1] 陈昆松[1]
机构地区:[1]浙江大学果实分子生理与生物技术实验室农业部园艺植物生长发育与生物技术重点开放实验室,杭州310029
出 处:《果树学报》2007年第6期778-782,共5页Journal of Fruit Science
基 金:国家自然科学基金(30571284);高等学校博士学科点专项科研基金(20040335022)
摘 要:以布鲁诺猕猴桃(Actinidia deliciosa cv.Bruno)成熟果实为材料,根据植物α-expansin(EXP)基因的氨基酸保守序列设计简并引物,利用RT-PCR方法结合3’RACE扩增得到1个长为957bp的cDNA片段(Ad-EXP1)。该基因片段编码211个氨基酸,与其它植物该基因核苷酸同源性为59%~85%,氨基酸同源性为73%~91%。Northern杂交结果表明,Ad-EXP1基因在采收当天的果实中表达很弱,随着果实成熟衰老进程加快趋于增强;乙酰水杨酸(ASA)处理延缓果实后熟软化和乙烯生成,也显著抑制Ad-EXP1基因表达。鉴于Ad-EXP1表达丰度与乙烯生成、果实软化程度的一致性,推测该基因在猕猴桃果实后熟软化进程中起着重要作用。A pair of degenerate primers was designed according to the conserved amino acid sequences from EXP (α-expansin) in other plants, and an EXP cDNA fragment was obtained from ripe kiwifruit (Actinidia deliciosa cv. Bruno) by incorporating RT-PCR and 3' RACE. The cDNA fragment, named as Ad-EXP1, was of 957 bp in size and encodes a peptide of 211 amino acids. The fragment showed high homologous to other known EXP genes, with sequence identity at 59% to 85% and 73% to 91% for nucleotide and amino acid levels, respectively. Northern blot analysis showed that the mRNA abundance of Ad-EXP1 was low at harvest, accumulated during fruit ripening and senescence. ASA treatment significantly retarded fruit softening, inhibited ethylene production, and reduced Ad-EXP1 mRNA abundance as well. Since the expression magnitude of Ad-EXP1 was consistent with the rate of ethylene production and the degree of fruit softening, this gene might be crucial in kiwifruit ripening and softening.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.171