中草药肉苁蓉和黄芪对猪早期胚胎体外发育的影响  

Effect of Chinese Herbal Medicine-cistanche and Radix Astragali on the Development of Early IVF Embryo

作　　者：李玉芳[1] 徐芹伟[2] 张丽[2] 张文昌[2] 冯书堂[3] 

机构地区：[1]黄淮学院,河南驻马店463000 [2]福建农林大学,福建福州350002 [3]北京畜牧兽医研究所,北京100094

出　　处：《江西农业大学学报》2007年第5期805-808,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金："十五"中国实验用小型猪资源开发与研究应用项目(2004BA717B);基础性工作;社会公益研究专项(2003DEB6J078)

摘　　要：利用猪体外受精的胚胎进行研究,比较了在培养基NCSU-23中添加中药成分肉苁蓉和黄芪对早期胚胎发育的影响,实验证明:(1)培养液中添加体积比0.10%的肉苁蓉和0.10%的黄芪,肉苁蓉添加组囊胚率(15.76%)显著高于空白组(9.26%)(P<0.05),黄芪添加组囊胚率(10.09%)与空白组(9.26%)差异不显著(P>0.05),表明肉苁蓉能够显著促进早期胚胎发育。(2)肉苁蓉添加浓度(V/V)为0.05%和0.10%时,囊胚率(17.02%和16.75%)显著高于空白组(0)(P<0.01)和0.15%组(8.94%)(P<0.05)。(3)培养液在2细胞期添加0.10%中药肉苁蓉,囊胚发育率(13.81%)显著高于4细胞期(7.53%)(P<0.05),比卵裂之前添加肉苁蓉(13.67%)稍高,但差异不明显(P>0.05)。The effect of Cistanche and Radix Astragali on the development of early IVF embryo was studied, the result suggested that, （1）The blatocysterate （15.76%） of the embryos cultured in the medium containing 0.10%（V/V） Cistanche and 0.10%（V/V）Radix Astragali was higer than that of the embryos cultured in the medium without Traditional Chinese Medicine markedly（9.26%）（P〉0.05）, and it was also a little higher than that of the embryos cultured in the medium containing Radix Astragali （10.09%）（P〉0.05）.（2）The blatocyst rates of the embryos culture d in the medium containing 0.05% and the medium containing 0.10%（V/V）Cistanche （17.02% ＆ 16.75%） were higher notablely than those of the embryos cultured in the medium containing 0.15%（V/V）Cistanche （0%）（P〈0.01） or the medium containing no Cistanche（8.94%）（P〈0.05）.（3）The blatocyst rate of the embryos transferred into the medium containing 0.01%（V/V）Cistanche at 2-cell time （13.81%） was significantly higher than that at 4-cell time （7.53%）（P〈0.05） and it was also a litter higher than that before cleavage （13.67%） （P〉0.05）.

关 键 词：卵母细胞 体外受精 肉苁蓉 早期胚胎 

分 类 号：S828.212[农业科学—畜牧学]