健脾与补肾对脾虚模型大鼠脑内生长抑素水平和受体基因表达的影响  

Influence of Invigorating Spleen and Restoring Virility to the SS Level and SSR1 Gene Expression in Brain of Spleen Insufficiency Model of Rat

作　　者：钱会南[1] 王乐[1] 胡雪琴[1] 沈丽波[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中医药信息杂志》2007年第11期23-24,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助(30171188)

摘　　要：目的比较健脾与补肾对脾虚模型大鼠脑内生长抑素(SS)水平和受体基因表达的影响。方法采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,以免疫组化和原位杂交法检测下丘脑腹侧核、海马CA1区、前额叶皮层SS及生长抑素受体1(SSR1)mRNA基因表达变化。结果模型组上述脑区SS免疫阳性反应物及SSR1 mRNA表达阳性反应物明显降低,与正常组比较差异显著;健脾组与补肾组上述脑区的SS免疫阳性反应物及SSR1 mRNA表达阳性反应物明显升高,与模型组比较差异显著,与正常组比较无显著差异。结论脾虚模型大鼠脑内对学习记忆有促进作用的SS水平和SSR1 mRNA基因表达有变化,健脾与补肾可能通过调节SS水平和SSR1基因表达而影响学习记忆能力。Objective To compare on the influence of invigorating spleen and restoring virility to the SS level and SSR1 gene expression in brain. Method The spleen insufficiency model of rat was established by adopting bitter decline diarrhea, wild eating and overworking. SS level and SSR1 gene expression change of hypothalarnus ventral kernel, hippocarnpi CA1 area and forehead corte were detected by immunohistochemistry and in situ hybridization techniques. Result In the model group, the immunoreaction masculine substance of SS and SSR1 mRNA, hypothalamus ventral kernel, hippocampi CA1 area and forehead cortex decreased obviously. The irnmunoreaction masculine substance of SS and SSR1 mRNA on above mentioned parts in the group of invigorating spleen and restoring virility increased distinctly. Conclusion In the model group, the SS level and SSR1 gene expression in brain to promoting study memory ability changed. Invigorating spleen and restoring virility may affect study memory ability by regulating the SS level and SSR1 gene expression.

关 键 词：脾虚模型 生长抑素 生长抑素受体 1 MRNA基因表达 大鼠 

分 类 号：R289.11[医药卫生—方剂学]