参附注射液对大鼠肢体缺血再灌注后髓过氧化物酶、丙二醛和能量代谢酶的影响  

Effect of Shenfu Injection on MPO,MDA and CCO,Ca^2＋-ATPaes after Ischemia/Reperfusion in Skeletal Muscle of Rats

作　　者：谢朝晖[1] 李岩[1] 严宛鸿[1] 王春爱[1] 周骁[1] 

机构地区：[1]甘肃省中医院,甘肃兰州730050

出　　处：《中国中医药信息杂志》2007年第11期37-38,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省自然科学基金资助（3ZS041-A25-070）

摘　　要：目的 观察参附注射液对大鼠肢体缺血再灌注后骨骼肌组织髓过氧化物酶（MPO）、丙二醛（MDA）含量和细胞色素氧化酶（CCO）及钙激活ATP酶（Ca^2＋-ATPaSe）活性水平变化的影响，探讨其作用机制。方法 SD大鼠30只，建立肢体缺血再灌注损伤模型。A组：对照组（10只），只做手术，不做阻断；B组：缺血再灌注组（10只），缺血4h，再灌注4h，未作药物治疗；C组：参附注射液治疗组（10只），缺血及再灌注时间同前一组。阻断前于股动脉阻断部位处注入参附注射液（2．0mL／kg），5min后夹闭股动静脉。再灌注前15min，再次自尾静脉注入同样浓度参附注射液，检测骨骼肌组织MPO、MDA、湿重／干重值（W／D）和CCO及Ca^2＋-ATPase活性的变化。结果 参附注射液能降低大鼠骨骼肌缺血再灌注损伤所引起的骨骼肌组织MPO、MDA、W／D的升高，在一定程度上保护CCO及Ca^2＋-ATPase的活性。结论 参附注射液可减轻肢体缺血再灌注时骨骼肌组织的损伤。Objective To observe the effect of shenfu injection on ischemia/reperfusion injury in skeletal muscle of rats. Methods Rat posterior limb ischemia/reperfusion model were made. Thirty SD rats were randomly divided into three groups （n =10）： sham group （group A）, ischemia/reperfusion group （group B） and shenfu injection treatment group（group C）, In group A, the rats received sham operation only. In group B, the animals recieved posterior limb ischemia 4 hours and reperfusion 4 hours. In group C, shenfu injection 2.0 mL/kg were injected iv 5 rain before ischemia and 15 min before reperfusion. The level of malondialdehyde （MDA）, myeloperoxidase （MPO）, wet weight/dry weight （W/D）, cytochrome oxidase （CCO） and Ca^2＋-ATPase in the skeletal muscle were measured at the end of the experiment. Shenfu injection can reduce the heightened level of MDA, MPO, W/D and protect the activity of CCO, Ca^2＋-ATPase in the skeletal muscle, lighten the injury of the ultrastructure. Condition Shenfu Injection can protect ischemia/reperfusion injury in skeletal muscle.

关 键 词：骨骼肌 再灌注损伤 参附注射液 大鼠 

分 类 号：R285.5[医药卫生—中药学]