白芥子不同炮制品中芥子碱硫氰酸盐含量测定  

Determination of Sinapine Thiocyanate in Semen Sinapis Albae of Different Processed Products

作　　者：刘起华[1] 张萱[1] 文谨[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国中医药信息杂志》2007年第11期46-47,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：中国中医科学院广安门医院资助课题(2004s77)

摘　　要：目的建立白芥子不同炮制品中芥子碱硫氰酸盐含量测定方法。方法采用RP-HPLC法,色谱柱为HypersilBDS苯基柱(5μm,250mm×4.6mm),以乙腈-0.08mol/L磷酸二氢钾溶液(15∶85)为流动相,检测波长240nm,流速为1.0mL/min。结果芥子碱硫氰酸盐进样量在0.8～4.0μg范围内线性关系良好,r=0.9997,平均回收率为100.98%(RSD=3.51%)。结论本法操作简便,结果准确,重现性好,可用于白芥子炮制品的质量控制。Objective To develop an HPLC method for the assay of sinapine thiocyanate in Semen Sinapis Albae of different processed products. Methods It was assayed by RP-HPLC. The column was Hypersil BDS Phenyl （5 μm, 250 mm×4.6 mm）, the mobile phase was acetonitrile-0.08 mol/L KH2PO4 （15 ： 85）. The UV detection wavelength was 240 nm. The flow rate was 1.0 mL/min. Results Good linearity of sinapine thiocyanate was showed within the range of 0.8～4.0 μg （r =0.9997）. The average recovery was 100.98% （RSD=3.51%）. Conclusion The method is simple, accurate and good reproducible. It can be used for quality control of Semen Sinapis Albae of different processed products.

关 键 词：白芥子 芥子碱硫氰酸盐 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]