郑州市肠出血性大肠杆菌O157:H7感染监测  

SURVEILLANCE FOR ENTEROHEMORRHAGIC E.COLI O157︰H7 IN ZHENGZHOU CITY

作　　者：殷毅峥[1] 程春荣[1] 杨建国[1] 安戈[1] 武恩平[1] 段晶晶[1] 水艳[1] 

机构地区：[1]郑州市疾病预防控制中心,郑州450000

出　　处：《现代预防医学》2007年第21期4175-4177,共3页Modern Preventive Medicine

摘　　要：[目的]2005年监测郑州市肠出血性大肠杆菌O157:H7感染状况及病原分布特点,为各级卫生行政部门制订新发传染病防治措施,提供基础疫情资料。[方法]监测标本用免疫磁珠集菌方法(IMS)做病原学分离培养、毒力基因检测用多重PCR方法、药敏试验用(K-B)常规方法。[结果]腹泻病人标本185份,大肠杆菌O157:H7检出阳性4份。外环境标本588份,检出大肠杆菌O157:H7阳性20份。用多重PCR方法做毒力基因检测,有6份家畜家禽阳性标本中检出O157:H7毒力基因(Stx2、eaeA、HIyA)为阳性。药敏试验24株肠出血性大肠杆菌O157:H7对8种抗菌药物敏感率为95%以上,对新生霉素100%耐药,其余7种对抗菌药物敏感程度各有差异。[结论]郑州市有散在的大肠杆菌O157:H7感染的病人及动物疫点。奶牛和波尔山羊是大肠杆菌O157:H7动物宿主。To monitor the infective state of enterohemorrhagic Escherichia coli O157︰H7 and etiologic distribution,in order to provide basic data about the epidemic situation for medical administrative departments to work out prevention and cure strategies for new infection diseases.[Methods]Specimens were isolated by IMS,virulence genic detection and drug sensitivity test were conducted by PCR and K-B.[Results]4 positive cases of Colibacillus O157︰H7 were deteced among 185 diarrhea patients. The virulence genes（stx2、eaeA、HiyA）were positive among 588 external environment samples. The sensitive rate of drug sensitivity test to 8 antibacterials was above 95%. The drug resistance rate was 100% to robiocina and the sensitive levels of the rest 7 kinds of antibacterials were different.[Conclusion]There is sporadic patients and animal epidemic focus which is infected by colibacillus O157︰H7. The cow and Bohr goat are animal host of colibacillus O157︰H7.

关 键 词：肠出血性大肠杆菌O157:H7 监测 免疫磁珠集菌法(IMS) 多重PCR法 药敏试验 

分 类 号：R378.21[医药卫生—病原生物学]