亚硝酸钠氧化法测定血清胆红素的质量评价  

Evaluation for Bilirubin Assay by Sodium Nitrite Oxidation Method

作　　者：尤涛[1] 邓克廷 田宇[1] 

机构地区：[1]西安医学院附属医院,陕西西安710077

出　　处：《实用医技杂志》2007年第22期2952-2953,共2页Journal of Practical Medical Techniques

摘　　要：目的:评价亚硝酸钠氧化法测定血清中总胆红素和直接胆红素。方法:测定精密性、准确性、抗干扰性及与改良J-G法的相关性。结果:总、直接胆红素的精密度分别为批内CV0.94%与0.98%,日间CV为1.1%和1.24%;平均回收率为总胆红素102.2%,直接胆红素98.1%;与改良J-G法的相关性为:总胆红素Y(亚硝酸钠)=0.959X(改良J-G) +0.186,r=0.992,直接胆红素:Y(亚硝酸钠)=1.006X(改良J-G)-0.091.r=0.991;血红蛋白浓度和甘油三酯在4 g/L和4.00mmol/L以下无干扰。结论:该法准确、稳定、简单、快速,完全可以满足临床应用。Objective Evaluation for sodium nitrite oxidation method measuring bilirubin. Methods Mensurate the accuracy and precision,interference experiment were made,The correlation analysis of sodium nitrite oxidation method and improved J-G method also were made. Result The CV of determination total and direct bilirubin was 0.94% and 0.98%,between day was 1.19% and 1.24% respectively.The average recovery was 102.2%for total bilirubin and 98.1% for direct bilirubin, The result of the method correlation well with improved J-G method. Theirs regression equation and r value were, TBIL Y （sodium nitrite）= 0.959X （J-G）＋ 0.186 ,r= 0.992, DBIL Y （sodium nitrite） = 1.006X （J-G）- 0.091 , r=0.991. HB〈4g/L and TG〈4.00mmol/L no interference was observed, Conclusion The method is accurate,stable,single,rapid,can be used in clinical area.

关 键 词：总胆红素 直接胆红素 亚硝酸钠氧化法 

分 类 号：R446.1[医药卫生—诊断学]