热休克蛋白27介导异氟醚预处理延迟相对缺血/再灌注损伤兔心肌的保护作用研究  

Protective effect of heat-shock protein 27 on myocardium with isoflurane preconditioning in myocardial ischemia/reperfusion injury of myocardium in rabbit

作　　者：冉珂[1] 朱蓉[1] 卢向航[1] 邹定全[1] 李辉[1] 常业恬[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,湖南长沙410011

出　　处：《中国危重病急救医学》2007年第11期683-686,共4页Chinese Critical Care Medicine

基　　金：湖南省自然科学基金资助项目（03JJY3053）

摘　　要：目的探讨热休克蛋白27（HSP27）在异氟醚预处理延迟相对缺血/再灌注（I/R）兔心肌保护中的作用机制。方法将30只新西兰大白兔随机分成假手术组（C组）、I/R组和体积分数为2%的异氟醚预处理组（S组）3组,每组10只。C组吸入纯氧2h,24h后仅行左冠状动脉（冠脉）套线而不阻断血流160min;I/R组吸入纯氧2h,24h后结扎左冠脉前降支阻断血流40min,再灌注120min;S组吸入2%的异氟醚和纯氧2h,24h后处理同I/R组。再灌注后抽血测定各组丙二醛（MDA）含量;用伊文思蓝和氯化三苯四唑（TTC）染色法测定心肌梗死面积;用蛋白质免疫印迹法（Westernblotting）测定各组HSP27和核转录因子-κB（NF-κB）的蛋白表达。结果异氟醚预处理延迟相可降低I/R损伤心肌梗死面积（19.7±2.8）%比（37.8±1.75）%,上调HSP27表达（84.5±4.3）灰度值比（53.1±3.8）灰度值,下调NF-кB表达（58.6±4.2）灰度值比（119.3±5.6）灰度值,降低MDA含量（5.24±0.45）kU/L比（9.42±0.83）kU/L,差异均有统计学意义（P均〈0.05）。结论HSP27介导了异氟醚预处理延迟相对I/R心肌的保护作用。Objective To investigate the mechanism and the protective effect of heart-shock protein 27 （HSP27） on rabbit myocardium with isoflurane preconditioning in myocardial ischemia/reperfusion （I/R） injury. Methods Thirty New Zealand white rabbits were randomly assigned to three groups （each n=10）：①Sham operation group （C group）; ②I/R group; ③Two percent in volume isoflurane group （S group）. S group was exposed to 2.0% isoflurane-pure oxygen for 2 hours. C group and I/R group were exposed 2 hours to pure oxygen to serve as untreated controls. Twenty-four hours later the rats in I/R group and S group underwent 40 minutes of coronary occlusion followed by 120 minutes of reperfusion. At the end of the reperfusion, infarct size （IS） was defined by Evan′s blue and triphenyltetrazolium chloride （TTC） staining. Blood samples were taken from arterial line for determination of malondialdehyde （MDA） levels. Western Blotting was used to determine the expression of HSP27 and nuclear factor-кB （NF-кB） in myocardium. Results Isoflurane preconditioning could decrease I/R induced myocardial infarct size （19.7±2.8）% vs. （37.8±1.7）%. This was accompanied by an increase in the expression in HSP27 （84.5±4.3） gray scale value vs. （53.1±3.8） gray scale value and a decrease in NF-кB （58.6±4.2） gray scale value vs. （119.3±5.6） gray scale value and MDA （5.24±0.45）kU/L vs. （9.42±0.83） kU/L. Conclusion The expression of HSP27 induced by isoflurane preconditioning plays an important role in protecting myocardium against ischemia/reperfusion injury.

关 键 词：异氟醚 预处理延迟相 缺血/再灌注损伤 心肌 热休克蛋白27 

分 类 号：R686[医药卫生—骨科学]