茵陈与熊去氧胆酸联用对妊娠肝内胆汁淤积症大鼠T辅助细胞因子Th1/Th2平衡状态及妊娠结局的影响  

Influences of Herba Artemisiae Scopariae Combined with Ursodesoxycholic Acid on the Balance Shift of Th1/Th2 and the Result of Pregnancy in Rats with Intrahepatic Cholestasis of Pregnancy

作　　者：胥银宇[1] 顾江红[1] 张信岳[2] 

机构地区：[1]杭州市中医院妇产科,浙江杭州310007 [2]浙江省医学科学院,浙江杭州310013

出　　处：《中华中医药学刊》2007年第11期2338-2340,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教委科研基金资助项目(20010044)

摘　　要：目的:探讨茵陈与熊去氧胆酸(UDCA)片联用对妊娠肝内胆汁淤积症(ICP)大鼠T辅助细胞因子Th1/Th2及妊娠结局的影响。方法:50只孕大鼠中任选40只诱发建立ICP模型,且随机分成4组。茵陈组10只孕鼠于妊娠第15天起用50%茵陈煎剂灌胃,5mL.d-1,连服10天;UDCA组10只孕大鼠于妊娠第15天起喂UDCA片,15mg.kg-1.d-1,连服10天;联合组10只孕大鼠于妊娠第15天起用50%茵陈煎剂灌胃,5mL.d-1,同时加用UDCA片灌胃,15mg.kg-1.d-1,连服10天;诱发后未治疗10只孕大鼠作为对照组。其余10只孕大鼠为正常组。应用酶联免疫吸附实验(ELISA法)测定50只孕鼠妊娠第15天(治疗前)和第21天血清中IL-2、IFN-γ、IL-4水平。记录各组孕大鼠妊娠天数及仔鼠存活率。结果:(1)ICP孕鼠(对照组)IL-2、IFN-γ水平高于正常组(P<0.05),IL-4水平低于正常组(P<0.05)。(2)茵陈组治疗后IL-2水平较治疗前、对照组下降(P<0.05);IL-4水平比治疗前、对照组上升(P<0.05);IFN-γ水平的变化无统计学意义(P>0.05)。UDCA组IL-2水平较治疗前、对照组下降(P<0.05);IL-4水平比治疗前、对照组上升(P<0.05);IFN-γ水平较治疗前下降(P<0.05),与对照组比较无统计学意义(P>0.05)。(3)联合组治疗后IL-2、IFN-γ水平较治疗前、对照组下降(P<0.05);IL-4水平比治疗前、对照组上升(P<0.05);且联合组IFN-γ水平较茵陈组、UDCA组低(P<0.05),各药物组的IL-2、IL-4水平比较无统计学意义(P>0.05)。(4)对照组孕鼠妊娠天数、仔鼠存活率与正常组比较(P<0.05)。治疗后各药物组妊娠天数与对照组比较明显延长(P<0.05),与正常组比较无差异(P>0.05);仔鼠存活率较对照组均明显提高(P<0.01),但茵陈组与UDCA组仍较联合组和正常组低(P<0.05),而联合组仔鼠存活率与正常组比较无差异(P>0.05)。结论:ICP孕鼠T辅助细胞功能发生了变化,Th1细胞因子过度表达导致Th1/Th2失衡。茵陈与UDCA联用对ICP孕鼠Th1/Th2平衡状态向Th2细胞因子转化及�Objective： To investigate the influences of herba artemisiae scopariae combined with ursodesoxycholic acid （UDCA） on the balance shift of Thl/Th2 and the result of pregnancy in pregnant rats with Intrahepatic Cholestasis of Pregnancy（ICP）. Methods： Model of ICP in pregnant rats was established and divided randomly into 4 groups. Herba ar- temisiae scoparlae group （10cases） treated with herba artemisiae scoparlae （5mL · d^-1） for 10 days. UDCA group （10cases） treated with UDCA （15mg·kg^-1·d^-1） for 10 days. Combination group （10cases） treated with herba arte- misiae scopariae （5mL · d^-1） and UDCA （15mg·kg^-1·d^-1） for 10 days. 10 pregnant rats with ICP were as control group. 10 normal pregnant rats were as normal group. Levels of interleukin -2 （IL -2）, interferon -gamma （IFN -y） and interleukin-4 （IL-4） in the serum were determined by using enzyme linked immunoabsorbent assay （ELISA） in 50 pregnant rats at 15th day （ befor treatment） and 21th day of pregnancy. To note the number of pregnant days and the survival rate of rat pups of each group. Results ： （ 1 ） Both the levels of IL - 2 and IFN - y in pregnant rats with ICP werehigher than those in normal pregnant rats （ P 〈 0.05）. Whereas the level of IL - 4 was lower （ P 〈 0.05）. （2） After the treatment with herba artemisiae scopariae or UDCA , the level of IL - 2 in pregnant rats with ICP was reduced signifi- cantly（ P 〈0.05）. Whereas the level of IL -4 was significantly increased （ P 〈0.05）. But the level of IFN -7 was not reduced significantly （ P 〉 0.05 ）. （ 3 ） The level of IFN - 7 and IL - 2 in combination group were reduced signifi- cantly after drug therapy（ P 〈 0.05）. Whereas the level of IL -4 was significantly increased （ P 〈 0.05）. The level of IFN -7 in combination group was lower than that in either herba artemisiae scopariae or UDCA group（ P 〈0.05）. The level of IFN - 7 and IL - 4 had no difference in three drug groups �

关 键 词：茵陈 熊去氧胆酸(UDCA) 妊娠肝内胆汁淤积症(ICP) Thl细胞因子 TH2细胞因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]