生物膜反应器脱氮性能及其硝化细菌的研究  

Study on Nitrogen Removal Performance of Biofilm Reactor and Nitrifying Bacteria in It

作　　者：张秀红[1] 周集体[1] 郭海燕[2] 曲宝成[1] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116023 [2]大连交通大学环境科学与工程学院,辽宁大连116028

出　　处：《化工环保》2007年第3期209-213,共5页Environmental Protection of Chemical Industry

摘　　要：采用模拟含氮废水对自行设计制作的A/O生物膜反应器进行脱氮性能研究。研究结果表明：在水温为20～23℃、水力停留时间为18h、进水总氮质量浓度为61～78m g/L、曝气量为25L/min、进水COD分别为110～165m g/L和205～272m g/L的条件下,废水的COD去除率均在90%以上,硝化率分别为98%和95%,总氮去除率分别为50%和78%。采用荧光原位杂交技术对反应器各区域中的硝化细菌进行了检测。结果表明,反应器中的硝化菌主要为亚硝化螺菌属和硝化螺菌属,有少量亚硝化单胞菌属和硝化杆菌属存在。亚硝化菌和硝化菌在好氧区数量相对较多,在缺氧区数量相对较少。The nitrogen removal performance of the self-devised-and-made biofilm reactor was investigated using simulated nitrogen-containing wastewater. Under the conditions of wastewater temperature 20-23 ℃, HRT 18 h, total mass concentration of the influent nitrogen 62 -78 mg/L, aeration rate 25 L/rain and influent COD 110 - 165 mg/L and 205 - 272 mg/L, the average removal rate of COD is above 90%, the nitrification efficiencies are 98% and 95% respectively, and the removal rates of total nitrogen are 50% and 78% respectively. The nitrifying bacteria in each zone of the reactor were analyzed by means of fluorescent in situ hybridization （FISH）. The results reveal that Nitrosospira sp. and Nitrospira sp. are the main nitrifying bacteria in the reactor, and a few of Nitrosomonas sp. and Nitrobacter sp. are also existed. The amount of ammonia oxidizing bacteria and nitrite oxidizing bacteria in the aerobic zone is more than that in the anoxic zone.

关 键 词：生物膜反应器 脱氮 荧光原位杂交 硝化细菌 

分 类 号：X703.3[环境科学与工程—环境工程]