1,25二羟基维生素D_3对大鼠脾调节性T细胞及其特异性转录因子表达的影响  

The effect of 1,25(OH)_2 VitD_3 on CD4^+ CD25^+ regulatory T cells and transcription factor Foxp3 in rat spleen

作　　者：李培[1] 祁晓平[1] 李刚[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2007年第3期129-131,136,共4页Parenteral & Enteral Nutrition

基　　金：国家重点基础研究发展规划项目资助(2003CB515502)

摘　　要：目的:探讨用1,25二羟基维生素D3[1,25(OH)2VitD3]预干预对大鼠辅助性T细胞(Th1)免疫应答中脾CD4+CD25+调节性T细胞及其特异性转录因子Foxp3基因表达的影响。方法:采用近交系大鼠,随机分为对照组、脂多糖(LPS)组和VitD3组。分别给予对照组和LPS组赋形剂,VitD3组用1,25(OH)2VitD3(0.25μg/只.d-1),连续灌胃14 d。第15天LPS组和VitD3组腹腔注射致死剂量LPS(10 mg/kg)。6 h后处死,用流式细胞仪检测脾CD4+CD25+调节性T细胞比例,用Realtime RT-PCR检测脾中Foxp3 mRNA的表达。结果:1,25(OH)2VitD3明显上调大鼠Th1免疫应答中脾CD4+CD25+调节性T细胞的比例及其特异性转录因子Foxp3 mRNA的表达。结论:1,25(OH)2VitD3预干预对大鼠Th1免疫应答中脾CD4+CD25+调节性T细胞及其特异性转录因子Foxp3的基因表达有显著影响。1,25(OH)2VitD3有可能通过促进CD4+CD25+调节性T细胞的发育,发挥其免疫调节作用。Objective： To investigate the effect of 1,25（OH）2 VitD3 on CD4^＋ , CD25^＋ regulatory T cells and its specific transcription factor Foxp3 in rat spleen in Th1 immune reactioin, Methods： Lewis rats were randomly divided into 3 groups. VitD3 group was fed with 1,25 （OH）2 VitD3 （0-25 p,g per rat a day） for 14 days. The control group and LPS group administered the isotonic NaCl in the same volume for the same long time. LPS （10 mg/kg） was administered intraperitoneally in LPS group and VitD3 group on the 15th day, and then the animals were sacrificed 6 hours later. The percentage of CD^＋ CD25^＋ regulatory T cells in lymphomonocytes in rat spleen was analyzed by Flow cytometry. The expression of Foxp3 gene in rat spleen was detected by Realtime RT-PCR. Results： 1,25 （OH）2 VitD3 increased the percentage of CD^4＋ CD^25＋ regulatory T cells in lymphomonocytes in rat spleen significantly. 1,25 （OH） 2 VitD3 also up regulated the expression of Foxp3 gene markedly. Conclusion： 1,25（OH）2 VitD3 has a great influence on CD^＋ CD25^＋ regulatory T cells and its specific transcription factor Foxp3. 1,25 （OH）2 VitD3 may enhance the development and function of CD4^＋ CD25^＋ regulatory T cells either directly or indirectly.

关 键 词：1 25二羟基维生素D3 CD4+CD25+调节性T细胞 特异性转录因子Foxp3 

分 类 号：R392[医药卫生—免疫学]