hMSCs成骨诱导前后免疫调节的实验研究  

Experimental study of Immunoregulation by DOC and hMSCs

作　　者：杨东明[1] 吴雪晖[1] 梁文杰[1] 王蒙[1] 罗飞[1] 许建中[1] 

机构地区：[1]第三军医大学附属西南医院骨科全军矫形外科中心,重庆400038

出　　处：《现代生物医学进展》2007年第11期1619-1622,共4页Progress in Modern Biomedicine

基　　金：国家高技术研究发展资助重大攻关课题("863"计划)(2006-AA205020)

摘　　要：目的:从免疫学机制方面探讨人骨髓间充质干细胞(Human bone marrow derived mesenchymal stem cell,hMSCs)、成骨诱导后骨髓间充质干细胞(Osteogenic cells differentiated from mesenchymal stem cells,DOC)免疫调节作用的可能机理。方法:hMSCs、DOC(成骨诱导6天、12天、18天),采用流式细胞技术(FACS),分别检测CD40、CD154、CD80、CD86、HLH-Ⅰ、HLA-Ⅱ在诱导前后其表达水平的变化。hMSCs和DOC(诱导18天),分别作混合淋巴细胞反应(CCK-8法),检测不同数量级hMSCs、DOC体外对双向混合淋巴细胞反应体系同种异体PBMCs增殖的影响,以及不同数量级hMSCs、DOC对经植物血凝素(PHA)刺激后同种异体PBMCS增殖的的影响。结果:hMSCs、DOC低水平表达CD40、CD154、CD80、CD86和HLA-Ⅱ,高水平表达HLA-Ⅰ。不同数量级hMSCs、DOC均可不同程度抑制同种异体PBMCs的增殖反应,其抑制效果大体与细胞量成正比,与阳性对照组相比,均有统计学差异(P<0.01)。结论:在体外实验中,hMSCs、DOC可能通过低免疫原性和降低T细胞的免疫应答从而维持其免疫调节功能。Objectives： To investigate the variation of factors related to immunology in order to explain possible low immunogenicity mechanism of osteogenic cells differentiated from mesenchymal stem cells（ Dec ） and human bone marrow derived mesenchymal stem cell（hMSCs）, and to investigate the outcome of inhibition of proliferation of allogenic PBMCs by Dec and hMSCs in order to explain its immunoregulation mechanism. Methods： The expressions of CD40, CD154, CD80, CD86, HLA-I and HLA-II in Dec （6, 12 and 18 days after induced differention） and undifferentiated hMSCs were detected by FACS analysis. Inhibition of the proliferation of allogenic PBMCs and allogenic PBMCs stimulated by PHA were detected with addition of different number of Dec （ 18 days atier differentiated） and undifferentiated hMSCs by two-way and one-way mixed lymphocyte culture （MLC） technique （CCK-8 method）, respectively. Results： Dec and hMSCs express CD40, CD154, CD80, CD86 and HLA-II at low level and express HLA-I at high level. Different number of Dec and hMSCs can suppress the proliferation of allogenic PBMCs in MLC system in a densi-ty-depended way. Compared to the positive control, there is statistical significance between them （P〈0.01）. Conclusion： With their low immunogenicity functions, Dec and hMSCs perform immunoregulatory functions by lowering the immunological response ofT cells in vitro.

关 键 词：HMSCS DOC 免疫原性 免疫调节 

分 类 号：Q78[生物学—分子生物学] Q813