那法瑞林长效制剂对大鼠垂体功能影响  

Effect of depot formulation of nafarelin on pituitary function in rats

作　　者：曹倩[1,2,3] 周美华[1,2,3] 王方敏 陆锦芳 

机构地区：[1]上海医科大学基础医学院药理教研室 [2]上海药品检验所 [3]上海医科大学药学院药剂教研室

出　　处：《中国药学杂志》1997年第6期340-342,共3页Chinese Pharmaceutical Journal

基　　金：高等学校博士学科点专项科研基金

摘　　要：目的：观察国产那法瑞林（ｎａｆａｒｅｌｉｎ）长效制剂的长效作用及对大鼠垂体功能影响，并探讨其作用部位。方法：采用大鼠间质细胞睾酮（ＲＩＣＴ）法检测血清ＬＨ（ｌｕｔｅｉｎｉｚｉｎｇｈｏｒｍｏｎｅ）生物活性水平。结果：那法瑞林长效制剂在体内的持续释药时间为（４０．０±６．８）ｄ。单次埋植３周后大鼠血ＬＨ的周期性分泌高峰及激发高峰均消失。预先埋植长效制剂组大鼠血ＬＨ在切除双侧卵巢后变化不明显。结论：那法瑞林长效制剂确有长效作用，其通过作用于垂体而抑制大鼠垂体功能。OBJECTIVE: To observe the duration and mechanism of action and effect of nafarelin (Naf) depot formulation on pituitary function in the rat. METHODS: A modified RICT (the rat interstitial cell testosterone) assay was used to determine serum bioactive LH levels of rats. RESULTS: The duration of action of Naf depot formulation in vivo was (40.0±6.8) d. The peak concentrations of serum LH levels in the estrous cycle and in the responses to an acute LHRH agonist challenge were suppressed significantly in the Naf depot formulation treated rats. No changes in serum LH levels before and after ovariectomy were observed in the rat previously treated with Naf depot formulation 2 weeks before ovariectomy. CONCLUSION: The long action of domestic Naf depot formulation is certain. The pituitary function of rats was suppressed directly and significantly 3 weeks after treatment with a single administration of Naf depot formulation.

关 键 词：那法瑞林 长效制剂 RICT法 

分 类 号：R977.1[医药卫生—药品]