多粘类芽孢杆菌HY96-2制剂中活菌的稳定性研究  

Studies on the Stability of Strain in Paenibacillus polymyxa HY96-2 Formulation

作　　者：李蜀[1] 李元广[1] 魏鸿刚[1] 王伟[1] 沈国敏[1] 李淑兰 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海泽元海洋生物技术有限公司,上海200237

出　　处：《农药》2007年第11期737-739,共3页Agrochemicals

基　　金：国家863项目(2006AA10A211);国家"十一.五"863项目(2006AA02Z222);上海市农业科技成果转化项目(0634H1N02);农业科技成果转化资金项目(04EFN213100093);上海市科委攻关项目(044319235)

摘　　要：考察了芽孢、贮存温度、含水率、光照和pH对多粘类芽孢杆菌HY96-2制剂稳定性的影响。结果表明,与非芽孢制剂相比,芽孢制剂在-20℃～37℃的范围内贮存时活菌存活率较高;含水率为5%左右的芽孢制剂的贮存稳定性较好;当含水率低于5%时,HY96-2芽孢制剂在-20℃贮存30d、20℃贮存90d和54℃贮存14d过程中,活菌存活率均在85%以上;光照2d后制剂中活菌的存活率仅为32.0%;pH在5.5～8时,HY96-2芽孢制剂中活菌能够正常地生长,并具有较高的活菌量。实际使用HY96-2芽孢制剂时,应选择阴天或傍晚,避开光照强烈的时间,并且施药土壤的pH宜在5.5～8范围内。The effect of spore-forming, store temperature, moisture content, sunlight and pH on biocontrol strain of Paenibacillus polymyxa HY96-2 formulation was studied. The results indicated that, comparing others with no spore, the cell viability of HY96-2 formulation with spore was higher when stored at -20 ℃ to 37 ℃, and the suitable moisture content was about 5%. The cell viability was more than 85% during storage at -20 ℃ to 30 days, 54 ℃ for 14 days and 20 ℃ for 90 days when the moisture content of HY96-2 formulation with spore was less than 5%. The cell viability was only 32.0% under sun light for one week. When the pH was 5.5 to 8, the cell of HY96-2 formulation with spore could grow normally and high cell concentration could get. So it was suitable when using HY96-2 formula- tion with spore that it was cloudy or on the evening for avoiding high sun illumination, and pH of soil was 5.5 to 8.

关 键 词：多粘类芽孢杆菌HY96-2 制剂 活菌存活率 稳定性 芽孢 

分 类 号：TQ450[化学工程—农药化工]