组织工程骨修复山羊胫骨节段性缺损的实验研究  

Repair of goat segmental tibial defects using tissue-engineered bone

作　　者：刘广鹏[1] 赵莉[1] 王衡健[1] 崔磊[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海组织工程研究与开发中心,200235

出　　处：《中华创伤骨科杂志》2007年第11期1053-1058,共6页Chinese Journal of Orthopaedic Trauma

基　　金：国家“973”组织工程基本科学问题项目（2005CB522701）;国家“863”组织工程骨构建技术研究与产品开发（2006AA02A123）;上海市科技启明星计划资助（07QA14053）

摘　　要：目的应用自体骨髓基质干细胞（BMSCs）复合β-磷酸三钙（β-TCP）构建组织工程化骨，修复山羊胫骨节段性缺损。方法体外扩增培养、成骨诱导山羊BMSCs。实验组将第2代细胞复合β-TCP后修复山羊自体右侧胫骨26mm的节段性缺损（n=8），对照组以单纯β-TCP材料植入骨缺损处（n=8），旷置组（n=2）。术后16、32周分别通过大体形态观察、影像学、组织学和生物力学的方法检测骨缺损的修复效果。结果旷置组术后32周骨缺损未修复，表明动物模型确实可靠。大体观察、X线片和MicroCT显示16周时实验组已有新骨形成，β-TCP材料降解吸收；对照组则只形成少量骨痂，材料无明显降解。组织学检测示实验组有大量幼稚编织骨生成，对照组为纤维结缔组织，并有大量材料残余。实验组骨密度和力学强度低于正常胫骨组（P〈0．05），但明显高于对照组（P〈0．01）。术后32周时大体观察X线片和MicroCT显示术后实验组骨愈合良好，对照组为骨不连；骨密度检测示实验组明显高于对照组（P〈0．05），且与正常胫骨组差异无统计学意义（P〉0．05）。组织学检测示实验组呈骨性愈合，有较多成熟骨组织，对照组为纤维连接。生物力学测试实验组与正常胫骨力学强度差异无统计学意义（P〉0．05）。结论成骨诱导的自体BMSCs复合β-TCP形成的组织工程骨可良好修复山羊胫骨节段性缺损。Objective To repair goat segmental tibial defects with tissue engineered bone constructed with autologous bone marrow stromal cells （BMSCs） and β-tricalcium phosphates （β-TCP）. Methods Isolated autologous BMSCs were in vitro expanded and osteogenically induced. In 18 goats, a 26 mm segmental defect at the right tibia was created. Eight defects were repaired with cell-scaffold constructs made from induced BMSCs and [β-tricalcium phosphate （[β-TCP）, another 8 defects with simple [β-TCP as control, and the remaining 2 defects were left untreated. The engineered bone was evaluated by X-ray, MicroCT, gross observation, histological examination, and biomechanical test at 16 and 32 weeks after operation respectively. Results The untreated 26 mm segmental defects did not heal. At 16 weeks, X-ray photographs showed obvious callus formation in the experimental group but a small amount of callus formation in the control group. At 32 weeks, gross observation, X-ray and MieroCT demonstrated good bony union in the experimental group but nonunion in the control group. A significantly higher bone mineral density was observed in the experimental group than in the control group. Histologically, mature bone and bony healing was commonly observed in the experimental group, while only fibrous healing in the control group. Biomeehanieal test revealed no significant difference between the 2 groups. Conclusion Goat segmental tibial defects can be well repaired with the tissue-engineered bone generated by β-TCP scaffold with autologous osteogenie BMSCs but cannot be repaired by β-TCP alone.

关 键 词：胫骨 缺损 骨髓细胞 磷酸钙类 组织工程 

分 类 号：R686[医药卫生—骨科学]