低能体外冲击波和低剂量间歇人重组甲状旁腺激素1-34刺激对体外培养大鼠成骨细胞增殖分化的影响  

Effects of stimulations by low density extracorporeal shock wave and low dose pulse recombined humen parathyroid hormone 1-34 on proliferation and differentiation of rat osteoblasts cultured in vitro

作　　者：刘长剑[1] 刘建国[1] 于铁成[1] 罗宗键[1] 郑学清[1] 

机构地区：[1]吉林大学附属一院骨科,长春130021

出　　处：《中华创伤骨科杂志》2007年第11期1062-1067,共6页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金资助项目（30500132）

摘　　要：目的探讨低能体外冲击波（ESW）和低剂量间歇人重组甲状旁腺素1-34（rhPTH1-34）对体外培养大鼠成骨细胞（ROBs）增殖和成骨分化的影响。方法分别用不同次数的低能ESW刺激、不同浓度及作用方式的rhPTH1-34刺激，以及ESW和低剂量间歇性rhPTH1-34刺激共同作用于ROBs后，用细胞计数、MTY和流式细胞术细胞周期分析检测ROBs的增殖，用酶标仪检测ALP活性，用免疫组化检测I型胶原表达来观察ROBs成骨分化。结果60～150次0．18mJ／mm^2 ESW刺激、间歇性rhPTH1-34（1×10^-11和1×10^-10 mol／L）刺激以及ESW＋间歇性rhPTH1-34（1×10^-11mol／L）刺激均可显著促进体外培养ROBs细胞增殖和成骨分化（与对照组比较，P〈0．05）；其中60～150次ESW刺激＋间歇性rhPTH1-34（1×10^-11 mol／L）刺激各组作用最强。结论适当的ESW应力刺激和低剂量间歇性rhPTH1-34刺激可显著促进体外培养ROBs细胞增殖和成骨分化，两者联合应用作用最强。Objective To investigate the effects of stimulations by low density extra corporeal shock wave（ESW） and low dose pulse recombined humen parathyroid hormone 1-34 （rhPTH1-34） on proliferation and differentiation of rat osteoblasts （ROBs） cultured in vitro. Methods After stimulations by 0.18 mJ/mm^2 ESW for different times, rhPTH1-34 of different patterns and ESW ＋ pulse rhPTH1-34 （ 1 × 10^-11 mol/L）, ROBs were collected to observe their proliferation by cell counting, MTT and DNA cycle analysis. Cell differentiation was observed by measuring ALP activity and type I collagen expression. Results Stimulations by 0. 18 mJ/mm^2 ESW （60-150 times）, pulse rhPTH1-34 （1 × 10^-11 and 1 × 10^-10 mol/L） and ESW （60-150 times） ＋ pulse rhPTH1-34 （1 × 10^-11 mod/L） all improved proliferation and differentiation of cultured ROBs （ P 〈 0.05, compared with control）. The effect of stimulation by ESW ＋ pulse rhPTH1-34 was stronger than that by ESW or pulse rhPTH1-34 （ 1 × 10^-11 tool/L） alone （ P 〈 0.05 ）. Conclusions Stimulations by suitable ESW and low dose pulse rhPTH1-34 can significantly improve proliferation and differentiation of ROBs cul- tured in vitro. The effect of ESW combined with pulse rhPTH1-34 （1 × 10^-11 mol/L） is the strongest.

关 键 词：体外冲击波 甲状旁腺激素类 鼠科 细胞周期 

分 类 号：R686[医药卫生—骨科学]