鼻息肉上皮细胞增殖活性和凋亡活性的比较  

Comparison between proliferation activity and apoptosis activity of epithelial cells in nasal polyps

作　　者：林心强[1] 王挥戈[1] 沈志忠[1] 张燕[1] 叶慧捷[1] 杨丹娜[1] 

机构地区：[1]汕头大学医学院第一附属医院耳鼻咽喉-头颈外科,广东汕头515041

出　　处：《山东大学耳鼻喉眼学报》2007年第5期430-432,435,I0002,共5页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：广东省医学科研基金课题(A1997321)

摘　　要：目的探讨鼻息肉上皮细胞增殖和凋亡平衡状态及其发病学意义。方法实验组(鼻息肉组)29例,对照组(下鼻甲组)11例,采用免疫组化染色法检测增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)的表达。采用TUNEL过氧化酶原位标记法检测原位细胞凋亡。结果①鼻息肉上皮PCNA阳性细胞指数(PIPCNA)和凋亡细胞指数(AI)均大于下鼻甲(30.02±5.89,5.33±2.79)(两者P<0.01),但鼻息肉上皮PIPCNA/AI值(1.95±0.66)小于下鼻甲(6.93±3.32)(P<0.01)。②鼻息肉上皮PIPCNA(53.60±11.10)大于AI(29.48±8.20)(P<0.01)。结论鼻息肉上皮细胞增殖活性和凋亡活性均增强,凋亡活性增强幅度较大,但总的来说,鼻息肉上皮细胞增殖率仍然大于细胞凋亡率,细胞累积增多,导致了鼻息肉上皮细胞的过度增殖。Objective To investigate the status of the balance between proliferation and apoptosis of epithelial cells in nasal polyps and to explore its pathogenic significance. Methods Expressions of PCNA were determined for 29 cases of nasal polyps in the treatment group and 11 cases of normal inferior turbinates in the control group by immunohistochemical staining. The apoptosis cells in site were determined by the TUNEL technique. Results （1） Both the PCNA positive index（PIPCNA）and the apoptosis index（AI）of epithelial cells in nasal polyps were prominently higher than those in normal inferior turbinates（30.02 ± 5.89, 5.33 ± 2.79, respectively）（ P 〈 0.01）. However, the PIPCNA/AI ratio of epithelial cells in nasal polyps（ 1.95 ± 0.66） was lower than that in normal inferior turbinates（6.93 ± 3.32） （ P 〈 0.01 ）. （2） The PIPCNA of epithelial cells in nasal polyps （53.60 ± 11.10） was prominently higher than the AI of epithelial cells in nasal polyps（29.48± 8.20）（ P 〈 0.01）. Conclusions Both proliferation actability and apoptosis actability of epithelial cells in nasal polyps are increasing, and the latter is of a large increasing range, but the proliferation rate of epithelial cells in nasal polyps, taking one with another, is still higher than the apoptosis rate, resulting in an increasing cell accumulation accompanied by over-proliferation of epithelial cells in nasal polyps.

关 键 词：鼻息肉 上皮细胞 增殖 凋亡 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]