大黄素可能通过抑制Akt信号通路诱导HL-60细胞凋亡  

Emodin induces leukemic HL-60 cells apoptosis probably by inhibiting Akt signal pathway

作　　者：郑合勇[1] 胡建达[1] 郑志宏[1] 黄绿叶[1] 陈英玉[1] 郑静[1] 陈鑫基[1] 吕联煌[1] 

机构地区：[1]福建医科大学附属协和医院福建省血液病研究所,福建福州350001

出　　处：《药学学报》2007年第11期1142-1146,共5页Acta Pharmaceutica Sinica

基　　金：福建省科技三项费用基金资助项目(2002Y048);福建省医学创新课题基金资助项目(2001CX02);福建医科大学教授基金资助项目(闽医大2006187);福建省高等学校新世纪优秀人才支持计划资助项目(NCETFJ-0604).

摘　　要：为研究大黄素(emodin)对人髓系白血病细胞株HL-60细胞增殖、凋亡的影响及Akt信号通路在其中的作用,应用MTT法检测大黄素对HL-60细胞增殖的影响;细胞周期分析、线粒体细胞凋亡流式检测法分析细胞周期变化及细胞凋亡;Western blotting检测Akt信号通路蛋白表达水平的变化。结果显示,大黄素能有效抑制HL-60细胞的增殖,作用48 h的IC50约为20μmol.L-1,并能诱导其凋亡,随药物作用浓度的增加,凋亡率也逐渐上升;大黄素作用后,HL-60细胞G0/G1期细胞增多,而S期及G2期的细胞减少;Western blotting检测结果显示,大黄素下调HL-60细胞Akt、p-Akt、IκB-α、p-IκB-α、p65、p-p65、mTOR及p-mTOR蛋白的表达。因此,大黄素能有效抑制HL-60细胞增殖,将细胞阻滞于G0/G1期,诱导其凋亡;Akt信号通路可能参与了大黄素抑制HL-60细胞增殖、诱导凋亡的过程。This study is to investigate the effect of emodin on inducing human myeloid leukemia cell line HL-60 apoptosis and the role of Akt signal pathway in the apoptosis. HL-60 cells were exposed to various dosages of emodin. MTT assay was used to detect HL-60 cell proliferation. Distribution of HL-60 cells in cell cycle was analyzed by flow cytometry and cell apoptosis was observed by MitoCapture apoptosis detection. The protein expressions of Akt signal pathway were detected by Western blotting. The result showed that emodin remarkably inhibited the cell proliferation. The IC50 value for 48 h treatment was about 20 μmol·L^-1 Apoptosis in HL-60 cells could be efficiently induced by emodin in a dose dependent manner and cells were arrested at G0/G1. The expressions of Akt、p-Akt、IκB-α、p-IκB-α、p65、p-p65、mTOR and p-mTOR in Akt signal pathway were downregulated after emodin treatment. It can be concluded that emodin could efficiently induce growth inhibition and apoptosis in HL-60 cells. Akt signal pathway may be involved in this process.

关 键 词：大黄素 HL-60细胞 细胞凋亡 AKT通路 白血病 

分 类 号：R963[医药卫生—微生物与生化药学]