芍药苷对佐剂性关节炎大鼠脾细胞产生抗体和cAMP水平的影响  

Effects of paeoniflorin on the level of antibodies and cAMP produced by splenocytes in rats with adjuvant arthritis

作　　者：陈尹[1] 魏伟[1] 吴虹[1] 张玲玲[1] 陈镜宇[1] 

机构地区：[1]安徽医科大学临床药理研究所,安徽合肥230032

出　　处：《药学学报》2007年第11期1147-1151,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30572356);安徽省自然科学基金资助项目(050430903);安徽省高校首批科技创新团队基金;高校博士点科研基金(20060366003).

摘　　要：探讨芍药苷(paeoniflorin,Pae)对佐剂性关节炎(adjuvant arthritis,AA)大鼠血清抗体水平和脾脏淋巴细胞cAMP水平的影响。大鼠足跖皮内注射弗氏完全佐剂(FCA)诱发大鼠AA模型,聚乙二醇(PEG)6000法检测血清循环免疫复合物(circulating immune complexes,CIC);酶联免疫吸附(ELISA)法检测血清中抗C II抗体(anti-C IIantibody)水平和抗结核杆菌抗体(anti-TB antibody)水平;放射免疫法(RIA)检测芍药苷体外给药对脾细胞中cAMP水平的影响。大鼠免疫后第17天开始分别灌胃给予芍药苷(25、50及100 mg.kg-1)和雷公藤多苷(40 mg.kg-1)对照药,连续给药7 d。芍药苷(50及100 mg.kg-1)可明显降低AA大鼠血清中升高的CIC水平、抗C II抗体水平和抗结核杆菌抗体水平,芍药苷组对AA模型组的抑制率(inhibition ratio,IR)与剂量呈正相关;芍药苷体外给药(12.5、62.5及312.5 mg.L-1)使AA大鼠脾细胞升高的cAMP水平降低。芍药苷治疗后,明显降低AA大鼠的血清抗体水平及大鼠脾细胞升高的cAMP水平。This study is to investigate the effects of paeoniflorin （Pae） on the levels of related serum antibodies and cAMP of splenocytes in rats with adjuvant arthritis. Complete Freund＇ s adjuvant was used to induce AA in rats. The level of circulating immune complexes in serum was determined by PEG6000 assay, and the levels of anti-C II antibody and anti-TB antibody in serum were measured by enzyme-linked immunosorbent assay （ELISA） , the level of cAMP in splenocytes was measured by radioimmunoassay, separately. Pae （25, 50, and 100 mg·kg^-1） and GTW （40 mg·kg^-1） were given by intragastric administration for 7 days from the 17th day after immunization. Pae （50 and 100 mg ·kg^-1 ） reduced the levels of circulating immune complexes, anti-C II antibody and anti-TB antibody in serum in rats with adjuvant arthritis. The inhibition ratios of Pae groups to AA model group were dosage-dependent; Pae （ 12.5, 62.5, and 312.5 mg ·L^-1 ） decreased the elevated levels of cAMP in splenocytes in vitro. Pae （ig） decreased the levels of related serum antibodies and elevated the level of cAMP in rats with adjuvant arthritis.

关 键 词：芍药苷 佐剂性关节炎 抗体 cAMP 

分 类 号：R282.71[医药卫生—中药学] R967[医药卫生—中医学]