盾叶薯蓣中水溶性甾体皂苷成分  

Isolation and structure determination of steroidal saponin from Dioscorea zingiberensis

作　　者：徐德平[1] 胡长鹰[2] 魏蕾[1] 庞自洁[3] 

机构地区：[1]江南大学食品学院,江苏无锡214036 [2]暨南大学食品科学与工程系,广东广州510520 [3]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《药学学报》2007年第11期1162-1165,共4页Acta Pharmaceutica Sinica

基　　金：江苏省发展与改革委员会资助（2004-419）,王林先生无偿提供盾叶薯蓣材料.

摘　　要：为研究盾叶薯蓣的水溶性化学成分,用溶剂提取,色谱法分离,光谱法鉴定结构。从盾叶薯蓣的水溶液中分离到5个甾体皂苷(I^V),经分析1HNMR、13C NMR、135DEPT、HMQC、HMBC和TOCSY谱,鉴定了化合物的结构,其中4个为原甾体皂苷,1个为甾体皂苷。化合物V为(25R)-26-O-(β-D-glucopyranosyl)-furost-5-en-3β,22ξ,26-triol-4β-acetoxyl-3-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside,为1新化合物,命名为zingiberenin G,化合物I、II、III在盾叶薯蓣中为首次报道。To study the chemical constituents of Dioscorea zingiberensis Wright, the EtOH extract of fresh rhizomes of D. zingiberensis was concentrated and partitioned further to produce petroleum ether-, ethylacetate-, n-butanol- and water-soluble fractions. The water-soluble fraction was subjected to column chromatography on macro resin AB-8, and the final products were obtained by repeated reversed-phase ODS and MCI gel CHP 20P column chromatography. Structures of compounds were elucidated by means of ^1H NMR, ^13 C NMR, ^135DEPT, HMQC, HMBC and TOCSY spectroscopic analyses. Five steroidal saponins were isolated, in which one new steroidal saponin was identified as （25R）-26-O-（β-D-glucopyranosyl）-furost-5-en-3β,22ξ,26-triol-4β-acetoxyl-3-O-β-D-glucopyranosyl-（1→3）-β-D-glucopyranosyl-（1→4）-[α-L-rhamnopyranosyl-（1→2）]-β-D-glucopyranoside （Ⅴ）.The compound V is a new compound, named as zingiberenin G. The compounds I, II and III were reported for the first time from D. zingiberensis Wright.

关 键 词：盾叶薯蓣 甾体皂苷 zingiberenin G 

分 类 号：R284.1[医药卫生—中药学]