光谱法研究CTMAB存在下荧光桃红B与fsDNA的相互作用及应用  

Spectrometric Study of the Phloxine B-fsDNA Interaction in the Presence of CTMAB and Its Application

作　　者：夏忠瑜[1] 李原芳[1] 龙云飞[1] 

机构地区：[1]西南大学化学化工学院

出　　处：《分析测试学报》2007年第6期851-854,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20425517;30570465)

摘　　要：研究了阴离子染料荧光桃红B（PB）对鱼精脱氧核糖核酸（fsDNA）和阳离子表面活性剂溴化十六烷基三甲铵（CTMAB）的共振光散射光谱有协同增强作用，建立了一种定量测定fsDNA的灵敏度高、操作简便的共振光散射（RLS）方法。在pH5．33和离子强度低于0．03mol／L的条件下，fsDNA与CTMAB和PB共同作用产生最大散射波长为340nm的特征共振光散射增强（RLSE）信号。在优化条件下，测定fsDNA的线性范围为0．01～3．0mg／L，检出限（36）为1．4μg/L。方法成功应用于合成样中DNA的测定。A highly sensitive resonance light scattering（RLS） method for the determination of fsDNA was developed. It was based on the enhancement of the intensity of RLS by the synergistical action of phloxine B（PB）, fsDNA and cetyltimethylammonium bromide（CTMAB）. At pH 5.33 and with ionic strength lower than 0.03 mol/L, the wavelength of the maximum intensity of RLS was 340 nm. Under optimum conditions, the linear range of the calibration curve was 0.01 - 3.0 mg/L with a detection limit of 1.4 μg/L. The method has been applied to the determination of DNA in synthetic samples with satisfactory results.

关 键 词：共振光散射 荧光桃红B 脱氧核糖核酸 溴化十六烷基三甲铵 

分 类 号：O657.3[理学—分析化学] Q512.8[理学—化学]