应用RAPD技术对大熊猫分类地位的探讨  

A PRELIMINARY STUDY ON THE TAXONOMY POSITION OF GIANT PANDA USING RAPD

作　　者：林峰[1] 杨玉华[1] 张义正[1] 陈红卫[2] 费立松[2] 宋云芳[2] 何光昕[2] 张安居[2] 

机构地区：[1]四川联合大学生物工程系分子生物学实验室 [2]成都动物园

出　　处：《兽类学报》1997年第3期161-164,77,共4页Acta Theriologica Sinica

基　　金：成都大熊猫繁育研究基金

摘　　要：采用ＰＣＲ和Ｓｏｕｔｈｅｒｎ杂交等方法对大熊猫与小熊猫、马来熊、浣熊等共有的一条１．３ｋｂ的ＲＡＰＤ产物片段进行了初步分析。研究结果显示，来自于大熊猫的此共有片段可能为一重复序列，并且其内部含有多个随机引物ＡＢ１－０８的结合位点。以此来自于大熊猫的１．３ｋｂ片段为探针进行杂交，发现马来熊ＲＡＰＤ产物中的对应片段显示了很高的同源性，而小熊猫和浣熊ＲＡＰＤ产物则无相应的杂交带。这暗示，从分类地位上来看，大熊猫与熊科马来熊的亲缘关系应更近于与小熊猫和浣熊的亲缘关系，应与马来熊一样划分为熊科。We employed PCR and Southern blot hybridization to analyze a shared 1 3 kb DNA fragment among the RAPD products from giant panda,lesser panda,malayan bear and raccoon genomic DNA.The results showed that the 1 3 kb fragment from giant panda may be a repetitive sequence,which contained several binding sites of the random primer AB1 08(GTCCACACGG).The southern blot hybridization result with the 1 3 kb fragment from giant panda as probe showed that the counterpart DNA fragment from malayan bear has strong hybridization signal while those from lesser panda and raccoon have no signal that could be detected.It suggested that giant panda might has closer relationship with bears than that with lesser panda and raccoon and should be classified into the Ursidae family with malayan bear.Our research result provide another evidence for the solution of the riddle of giant panda taxonomy position on molecular biology level.

关 键 词：大熊猫 RAPD分析 分类学 杂交 

分 类 号：Q959.838[生物学—动物学]