医药用神经节苷脂的制备方法  

A method for preparation of ganglioside used in medicine

作　　者：李钦选[1] 王素琴[1] 王志 赵云生[1] 李东亮[1] 马国军[1] 

机构地区：[1]新乡医学院,河南新乡453003 [2]洛阳市建工医院

出　　处：《新乡医学院学报》1997年第1期46-49,共4页Journal of Xinxiang Medical University

基　　金：河南省科委科技攻关项目

摘　　要：目的探索医药用神经节苷脂的最优（纯度）、成本低）生产方法。方法牛脑的氯仿-甲醇提取液通过水草取使神经节苷脂与脂溶性成分分离、再经碱水解除磷脂，上吸附树脂柱，使与水溶性杂质分离。然后作产品鉴定（包括纯度、组成成分、含磷量、活性试验及蛋白反应）。结果所制备的神经节苷脂的纯度为唾液酸含量26％；组成成分：GM116％、GD36％、GD1α／45％、GD1b10％、GT1α14％、GT1b17％；含磷量120μg／100mgGLs；生物活性：较好地促进离体鸡胚背根神经节的存活与生长。结论本法制备的神经节苷脂纯度高、成本低适用于医药用神经节苷脂的生长。Objective To research an optimal method of preparing ganglioside used in medicine with a good purify and low consumption fo for batch procuce.Methods By means of extraction with water for chloroform- methanol extracte of bovine brain, the gangliosides was isolated from the 1ipid-soluble substances.The phospho1ipids in theextracte solution was remove by aldaline hydrolysis, then the adsorptinn resin column chromatography was carried out to remove the water-soluble substances. The purity,component, phophatide content, biology active of the ganglioside were evaluated.Result The composition of ganglioside prepa-ration was showed by HPTLC are the follows16% GM,, 6% GD,, 45% GD 10% GD,b'14% GT 17 % GT,b' It contains 26% sinllc acid Per 100mg of gangliosides 120μg of phospholipids. The preparation showed a biology activity of promoting survival and groWth of dor-sal root ganglin in vitro.Conclusion This method is suitable to pre-paring ganglioside used in medicine for its good purity and the lowest comsumption.

关 键 词：神经节苷脂 制备 医药 牛脑 唾液酸 

分 类 号：R392.33[医药卫生—免疫学] R338[医药卫生—基础医学]