套式聚合酶链反应加限制酶谱分析检测母婴垂直传播巨细胞病毒  

Application of Nested Polymerase Chain Reaction (PCR) to the Study of HCMV Maternal-fetal Transmission

作　　者：程永璋[1,2] 尚世强[1,2] 洪文澜[1,2] 余钟声[1,2] 潘存梅[1,2] 俞锡林[1,2] 石一复[1,2] 顾佩宝[1,2] 

机构地区：[1]浙江医科大学儿童医院 [2]浙江医科大学妇产科医院

出　　处：《中国冶金工业医学杂志》1997年第2期84-86,共3页Chinese Medical Journal of Metallurgical industry

基　　金：浙江省自然科学基金;卫生厅资助

摘　　要：目的：克服一次性ＰＣＲ假阳性及非特异性条带干扰。方法：建立套式ＰＣＲ技术，对１０５份母－脐配对血进行套式ＰＣＲ、病毒分离、特异性ＩｇＭ以及特异性ＩｇＡ测定。结果：母血ＨＣＭＶＤＮＡ阳性６份，脐血３份，母－脐传播率为３／６。３对被证实为母婴垂直传播ＨＣＭＶ的标本中，２对套式ＰＣＲ、病毒分离、特异性ＩｇＭ及ＩｇＡ均阳性，１对套式ＰＣＲ病毒分离，特异性ＩｇＡ阳性，但特异性ＩｇＭ阴性。结论：套式ＰＣＲ是一种敏感特异、简便快速、能早期诊断ＨＣＭＶ的检测手段，适用于孕妇及胎儿ＨＣＭＶ感染的监测。Objective:To overcome the interruption of false positive and undesirable nonspecific banding with single PCR.Methods:Nested PCR was developed.One hundred and five pairs of maternal-infant umbilical blood were analysed by nested PCR,virus isolation and specific antibodies.Results:Six samples of maternal blood were positive with human cytomegalovirus (HCMV) DNA,3 matched umbilical blood were positive also.The rate of maternal-fetal transmission was 3/6.Of the 3 matched pair blood samples considered as HCMV intrauterine infection,2 pairs were HCMV positive with nested PCR,virus isolation,specific IgM and IgA.One pair was nested PCR,virus isolation,specific IgA positive and specific IgM negative.Conclusions:Nested PCR,being sensitive,specific rapid and simple,was available for the detection of HCM in pregnant women and fetuses.

关 键 词：巨细胞病毒 聚合酶链反应 垂直传播 母婴传染 

分 类 号：R722.13[医药卫生—儿科]