健中愈疡片对胃溃疡线粒体DNA修复酶调控机制的实验与临床研究  

The Clinical and Experimental Study of Adjustment of Mitochondrion DNA Repair Enzyme of Gastric Ulcer by JianZhongYuYang Tablet

作　　者：姬爱冬[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405

出　　处：《时珍国医国药》2007年第11期2595-2599,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金资助项目(No30371706)

摘　　要：目的从分子水平探讨健脾益气方药在胃溃疡黏膜损伤修复过程中对线粒体DNA修复酶的调控机制及观察愈合质量,深入探讨线粒体DNA修复酶对胃溃疡的胃黏膜的修复逆转作用及中药的作用靶点。方法用临床观察和动物实验研究健脾益气方药对胃溃疡胃黏膜的保护与修复作用。比较健中愈疡片、黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃黏膜线粒体8-氧鸟嘌呤DNA糖基化酶、胸腺嘧啶乙二醇DNA糖基化酶、3-甲基腺嘌呤DNA糖基化酶的影响,并进行健脾益气方药与黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃溃疡愈合质量的比较和评价。结果正常组线粒体DNA修复酶含量很低,胃溃疡组线粒体DNA修复酶含量很高,健中愈疡片组线粒体DNA修复酶含量较正常组极显著增高(P<0.01),胃舒平组线粒体DNA修复酶含量与胃溃疡组没有差异(P>0.05),法莫替丁组线粒体DNA修复酶含量比胃溃疡组减少(P<0.05)。而溃疡愈合质量则健中愈疡片组和法莫替丁组没有差异(P>0.05),二者均好于胃舒平组(P<0.05);在脾虚证的改善上,健中愈疡片组明显优于法莫替丁组和胃舒平组(P<0.01)。结论健中愈疡片对胃溃疡的治疗机理之一是对线粒体DNA修复酶进行了调控,提高了修复因素,这是其作用靶点之一;而法莫替丁和胃舒平组治疗机理分别是减少损害因素和进行物理保护,其作用与线粒体DNA修复酶关系不甚密切;但对脾虚症状的改善上,健中愈疡片的作用远远高于西药组,显示了中医药治疗的优越性。Objective Clinical practice has proved that invigorating spleen and benefiting vital energy Chinese formulated products -Jian Zhong Yu Yang Tablet（JZYYT） has good curative effect on tough gastric ulcer. Our research is to reveal how the invigorating spleen and benefiting vital energy Chinese drugs regulate the mitochondrion DNA repair enzymes, to reveal how Chinese drugs cure gastric ulcer, and to find the target of Chinese drugs. Methods Our study was to treat gastric ulcer by invigorating spleen and benefiting vital energy Chinese drugs through clinical observation and experimental research, and compared with mucosa protectant - gastropine and H2 receptor antagonist - famotidine about the mucosa mitochondrion 8 - oxygen guanine DNA glycosylase, thymine glycol DNA glycosylase, 3 - methyl adenine DNA glycosylase, the effect of gastralgia, splenic asthenia and quality of ulcer recovery. In clinical research, we observed the rank of gastralgia, splenic asthenia and compositive therapeutic effects. In animal experiment, we detected the body weight, frequency of twisting in unit time, suspending time, index number of gastric ulcer, and microcirculation of gastric mucosa such as the value of B/（ R ＋ G ＋ B） to clarify the regulation effect of invigorating spleen and benefiting vital energy Chinese drugs on mitochondrion DNA repair enzymes, and the therapeutic effect on gastric ulcer in molecular level. Results Mitochondrion DNA repair enzymes were the lowest in the gastric mucosa of normal group, but they were very high in gastric ulcer group, there had significant difference between them（ P 〈 0.01 ） ; Mitochondrion DNA repair enzymes had no difference between gastropine group and gastric ulcer group（ P 〉 0.05 ） ; Mitochondrion DNA repair enzymes in famotidine group decreased extremely compared with gastric ulcer group（ P 〈 0.01 ）, but still extremely higher than normal group（ P 〈 0.01 ） ;Mitochondrion DNA repair enzymes were the highest in JianZhongYuYang Tablet group, and had extr

关 键 词：健中愈疡片 胃溃疡 线粒体DNA修复酶 

分 类 号：R285.35[医药卫生—中药学] R285.6[医药卫生—中医学]