青刺果黄酮对糖尿病小鼠肝组织病理变化的影响  

Effect of Flavonoids from Prinsepia utilis Royle on the Histomorphology of Liver in the Diabetic Mice

作　　者：吴小兰[1] 殷中琼[1] 贾仁勇[1] 刘定帮[2] 杜永华[1] 陈建[1] 宛燕飞[1] 熊翼[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014 [2]四川省长江造林局,四川宜宾644002

出　　处：《时珍国医国药》2007年第11期2661-2663,共3页Lishizhen Medicine and Materia Medica Research

基　　金：企业横向合作资助项目

摘　　要：目的观察青刺果黄酮(FPR)对糖尿病小鼠肝组织病理变化的影响。方法用四氧嘧啶腹腔注射建立糖尿病小鼠模型,给予青刺果黄酮(300mg.kg-1)灌胃治疗,连续4周。分别于2周和4周末各组随机抽取4只处死,取肝脏称重,然后用4%的多聚甲醛固定。肝组织石蜡切片,HE染色,显微镜观察。结果糖尿病(DM)小鼠肝脏指数显著增加,肝细胞失去正常的索状结构,肝窦变窄,肝细胞索之间纤维细胞增生。肝细胞肿胀,发生颗粒变性,中央静脉周围的肝细胞发生脂肪变性,严重者,肝细胞出现多灶性坏死灶,坏死灶内细胞核浓缩、碎裂、溶解,淋巴细胞和中性粒细胞浸润。中央静脉和小静脉淤血。FPR治疗组,肝脏指数显著降低,肝细胞的索状结构清楚,肝窦较正常,肝细胞呈轻微的颗粒变性。结论FPR能抑制糖尿病小鼠肝脏肿大,减轻肝脏组织病理变化,有效延缓糖尿病引起的肝脏损伤。Objective To investigate the effect of llavonoids from Prinsepia utilis Royle on the histomorphology of liver in diabetic mice. Methods Diabetic mice induced by alloxan were given flavonoids from prinsepia utilis royle orally 300 mg · kg^-1 each day for four weeks. Two weeks and four weeks later four mice in each group were randomly sacrificed, livers were taben out and the weights were measured, and then fixed with 4% polyoxym ethylene. The hepatic tissue was embedded with paraffin, sectioned, and stained with Hematoxylin and Eosin（ HE）, the histopathology was observed with microscope. Results Compared with normal control group, as for the model control group, hepatic index of mice markedly increased, liver cell cords were disordered or disappeared, liver antrum became more narrow, fibrocytes proliferated between liver cell cords; hepatic cells were swollen and developed granular degeneration, hepatic cells around central vein developed fatty degeneration, some serious, hepatic cells appeared many necrotic areas, cellular nucleus concentrated, fragmentated, dissolved in necrotic areas, lymphocytes and neutrophils infiltrated in necrotic areas; central vein and small vein stagnanted blood. While compared with model control group, in the treatment group, hepatic index of mice markedly decreased, liver cell cords clear, liver antrum almost recovered to normal, hepatic cells developed slightly granular degeneration. Conclusion Flavonoids from Prinsepia utilis Royle can inhibit hepatic hypertrophy, relieve the changes of hepatic pathology,and delay the progression of injury of liver induced by diabetes mellitus.

关 键 词：青刺果黄酮 糖尿病 肝脏 组织形态 

分 类 号：R962[医药卫生—药理学]