酪蛋白对高碘小鼠甲状腺细胞凋亡的影响  

Effects of casein on apoptosis of mice thyroid under excessive iodine

作　　者：李颖[1] 刘守军[1] 于钧[1] 张智毅[1] 张树彬[1] 叶永祥[1] 苏明[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心碘缺乏病防治研究所,150081

出　　处：《中国地方病学杂志》2007年第6期616-619,共4页Chinese Jouranl of Endemiology

基　　金：黑龙江省教育厅课题(11511216);黑龙江省卫生厅课题(2005-003)

摘　　要：目的观察酪蛋白(CA)对高碘小鼠甲状腺细胞凋亡的影响。方法将昆明种小鼠随机分为4组:对照(NI)组、高碘(HI)组、高碘+CA1(HI+CA1)组、高碘+CA2(HI+CA2)组,喂养12个月,ELISA法检测血清白细胞介素1β(IL-1β),RT-PCR法检测甲状腺组织Fas、FasL mRNA表达水平,TUNEL法检测甲状腺细胞凋亡。结果与NI组比较,HI组小鼠血清IL-1β水平和甲状腺组织Fas mRNA表达均明显增高(P<0.05或<0.01),且甲状腺滤泡上皮凋亡细胞数也明显增多(P<0.01),而高碘小鼠随着CA摄入量增高,上述改变程度明显降低。结论高碘可通过提高机体IL-1β水平,诱发凋亡调控基因Fas/FasL系统表达改变,促进大鼠甲状腺细胞凋亡。而CA摄入可以部分拮抗高碘引起的上述改变,对滤泡上皮细胞具有一定的保护作用。Objective To observe the effects of casein （CA） on apoptosis of mice thyroid under excessive iodine. Methods Kunming mice were divided into 4 groups： normal control（NI） group, high iodine（HI） group, high iodine plus CA1 （HI ＋ CA1 ） group and high iodine plus CA2 （HI ＋ CA2） group. After twelve months, we examined the levels of IL-1D in the serum by ELISA, thyroid apoptosis by TUNEL, and Fas, FasL mRNA by RT- PCR. Results Compared with that in NI, the levels of IL-1β in the serum, Fas mRNA exprsssion and cell apoptosis of thyroid increased markedly in HI（P 〈 0.05 or 〈 0.01 ）, however they decreased in the high iodine plus CA group. Conclusions Iodine excess may elevate IL-1β in the serum and stimulate Fas/FasL system expression of thyroid follicles epithelial cell, leading to thyroid cell apoptosis, which can be protected by CA protein.

关 键 词：碘 酪蛋白类 甲状腺 白细胞介素1 FAS基因 FASL基因 

分 类 号：R581[医药卫生—内分泌]