一个嫁接有羧酸羟基乙基酯的二吡啶吩嗪Ru(Ⅱ)配合物的合成、与DNA的相互作用以及溶剂变色性质的研究  

Study on the Synthesis, DNA Interaction, and Solvatochromism of a Hydroxyethyl Carboxylate-Grafted Dipyridophenazine Ruthenium(Ⅱ) Complex

作　　者：韩洋[1] 杨维春[1,2] 王科志[1] 

机构地区：[1]北京师范大学化学学院 [2]平顶山学院化学化工学院平顶山467002

出　　处：《化学学报》2007年第21期2382-2386,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.20371008;90401007);北京市自然科学基金(Nos.2052008;2072011);高等学校博士学科点专项科研基金(No.20060027002)资助项目.

摘　　要：合成并表征了一个新的Ru(II)配合物[Ru(bpy)2(hedppc)](ClO4)2{bpy=2,2'-联吡啶,hedppc=二联吡啶[3,2-a:2',3'-c]吩嗪-11-羧酸(2-羟乙基)酯}.通过紫外-可见吸收光谱、与溴化乙锭竞争实验、粘度测量和DNA裂解实验研究了配合物与小牛胸腺DNA的相互作用性质.结果表明配合物以插入模式与DNA键合,键合常数Kb=(6.99±1.34)×106mol-1?L(s=2.03±0.04)与母体配合物[Ru(bpy)2(dppz)]2+相近,但光致发光和溶剂变色等光学性质与[Ru(bpy)2(dppz)]2+有明显的差别.A new ruthenium（II） polypyridyl complex of [Ru（bpy）2（hedppc）]（ClO4）2 {in which bpy= 2,2′-biyridine, hedppc=2-hydroxyethyl dipyrido[3,2-a：2′,3′-c]phenazine-ll-carboxylate} was synthesized and characterize.d by elemental analysis, ^1H NMR and MALDI-TOF MS. The binding of the complex to calf thymus （ct） DNA was studied with UV-visible absorption and luminescence spectrophotometric titrations, DNA competitive binding with ethidium bromide, viscosity measurements and thermal denaturation experiments. The results indicated that the complex was bound to ct-DNA through an intercalative mode with an intrinsic binding constant of （6.99± 1.34）× 10^6 L/mol in buffered 50 mmol/L NaCl comparable to parent [Ru（bpy）2（dppz）]^2＋. The complex is permanently non-emissive in the absence and presence of the DNA, and exhibits intriguing solvatochromic properties different from parent [Ru（bpy）2（dppz）]^2＋.

关 键 词：钌(Ⅱ) DNA 紫外-可见吸收光谱 溶剂变色 

分 类 号：O641.4[理学—物理化学]