水稻亚种间基因组水平微卫星多态性分析  

Analysis of the whole-genome-wide polymorphisms of microsatellites between two rice subspicies

作　　者：谭军[1] 徐勤民[1] 周其兴[2] 薛庆中[3] 

机构地区：[1]重庆邮电大学生物信息研究所,重庆400065 [2]大连大学生物工程学院,辽宁大连116622 [3]浙江大学农业与生物技术学院,杭州310039

出　　处：《重庆工商大学学报（自然科学版）》2007年第1期16-21,共6页Journal of Chongqing Technology and Business University:Natural Science Edition

基　　金：国家自然科学基金资助项目(3030021);重庆市自然科学基金资助项目(2005BB5053)

摘　　要：从公布的水稻两个亚种(籼稻9311和粳稻Nipponbare)基因组草图序列中,搜寻了所有的完整微卫星位点,发现单碱基重复中A/T重复占大多数,二碱基重复中AT/TA、GA/TC、AG/CT重复居多,三碱基中GGC/GCC、GCG/CGC、CCG/CGG重复较多。通过相互比较,不同基序和基因区域的微卫星多态性有较大差异:内含子和基因间区中微卫星的多态性较高(分别约为45%和40%),外显子中的微卫星多为三碱基重复,而且多态性较低(约为25%);两个亚种具多态性的微卫星间距平均约为12.7 kb和16.4 kb。　All perfect microsatellites in draft genome sequences of two main rice subspecies（indica ssp.9311 and japonica ssp.Nipponbare） were detected in this paper.Most of MNRs are A/T repeats;most of the dinucleotide repeats（DNRs） are AT/TA,GA/TC,AG/CT repeats;most of trinucleotide repeats（TNRs） are GGC/GCC,GCG/CGC,CCG/CGG repeats.Through comparison,the polymorphisms of microsatellites of different gene sequences and gene regions have bigger difference.Microsatellites in introns and in intergenic regions are with polymorphism rate around 45% and around 40% respectively.Microsatellites in exons are mainly composed of trinucleotide repeats and have the lowest polymorphism rate（around 25%）.The distances of detected polymorphism microsatellites in the two subspecies are 12.7kb and 16.4kb respectively.

关 键 词：水稻亚种 基因组 微卫星 多态性 分子进化 

分 类 号：S530[农业科学—作物学]