有齿食道口线虫msp基因的克隆与表达  

Cloning and Expression of Major Sperm Protein Gene from Oesophagostomum dentatum

作　　者：林瑞庆[1] 庄瑞宏[1] 张雪[1] 林肖玲[1] 宋慧群[1] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2007年第4期91-94,共4页Journal of South China Agricultural University

基　　金：国家杰出青年科学基金(30225033);教育部优秀青年教师资助计划项目(教人司[2002]350号)

摘　　要：以有齿食道口线虫为研究对象,扩增出其雄虫的msp基因,克隆至质粒载体pTrcHis-B中,构建了msp基因重组原核表达载体.经测序表明,目的基因msp已以正确的阅读框架整合至表达质粒中.应用大肠杆菌Top10为宿主菌,通过IPTG诱导方法,表达包含msp基因产物的融合蛋白,经SDS-PAGE分析,表明表达蛋白大小正确,表达量高.The major sperm protein（MSP） gene of Oesophagostomum dentatum was amplified by PCR and subsequently inserted into the expression vector pTrcHis-B.The msp gene was sequenced and compared with other published msp gene sequences.Sequence analysis revealed that the reading frame of the msp gene inserted was correct.The MSP fusion protein was expressed in Escherichia coli（Top10） after induction with IPTG.SDS-PAGE analysis showed that the msp fusion protein was expressed efficiently.

关 键 词：有齿食道口线虫 msp基因 克隆 表达 

分 类 号：S852.73[农业科学—基础兽医学] Q78[农业科学—兽医学]