H5亚型禽流感病毒血凝素蛋白单克隆抗体的制备及鉴定  

Preparation and Identification of Monoclonal Antibodies Against Haemagglutinin of Subtype H5 Avian Influenza Virus

作　　者：谢青梅[1] 徐维加[2] 陈俊伟[1] 王玲玲[1] 马静云[1] 毕英佐[1] 曹永长[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]云南出入境检验检疫局,云南昆明650228

出　　处：《华南农业大学学报》2007年第4期117-118,共2页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2005A20901004)

摘　　要：用纯化的H5亚型禽流感病毒(AIV)抗原免疫Balb/C小鼠,取免疫小鼠脾细胞与SP2/0骨髓瘤细胞融合,经筛选,获得9株稳定分泌单克隆抗体的杂交瘤细胞株,其中4株能高效分泌血凝素(HA)蛋白特异性的单克隆抗体.特异性试验表明,IE6单克隆抗体仅与试验的H5病毒株反应,而不与新城疫病毒(NDV)、H9亚型AIV和产蛋下降综合症病毒(EDSV)反应.这9株单克隆抗体分别为IgG1、IgG2a、IgG2b、IgM亚类,κ轻链.Balb/C mice were immunized with the purified H5N1 and H9N2 subtype avian influenza virus（AIV） antigens.And monoclonal antibodies against above antigens were produced.Splenocytes from the immunized mice were fused with SP2/0 myeloma cells.Positive hybridoma clones were screened by ELISA,and nine monoclonal antibodies were generated following conventional protocols for making monoclonal antibodies.The nine monoclonal antibodies reacted with newcastle disease virus（NDV）,H5 subtype and H9 subtype AIV antigen,and only IE6 was specific to H5 subtype AIV.And IIB6C3,IIA8H11,IE6,IIIC2D1 were specific to the hemagglutinin（HA） fusion proteins.The nine monoclonal antibodies belonged to the IgG1、IgG2a、IgG2b、IgM subclass,with κ type light chain.

关 键 词：H5亚型 禽流感病毒 血凝素蛋白 单克隆抗体 

分 类 号：S852.659.5[农业科学—基础兽医学]