依曲替酸和窄谱中波紫外线对正常人角质形成细胞维甲酸受体γ mRNA表达的协同影响  

Synergistic effects of acitretin and narrow-band ultraviolet-B in inducing retinoic acid receptor gamma mRNA expression in normal human keratinocytes

作　　者：罗素菊[1] 彭振辉[1] 郑焱[1] 徐浩翔[1] 周少娜[1] 李晓莉[1] 王国荣[2] 

机构地区：[1]西安交通大学第二医院皮肤科,陕西西安710004 [2]陕西省人民医院普通外科,陕西西安710068

出　　处：《南方医科大学学报》2007年第11期1631-1634,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30371295;30400387);陕西省自然科学基金(2004C211)~~

摘　　要：目的研究依曲替酸和窄谱中波紫外线(NB-UVB)对培养的正常人角质形成细胞(KC)增殖和维甲酸受体γ(RARγ)mRNA表达的影响。方法用10-6mol/L依曲替酸和/或100mJ/cm2NB-UVB处理正常人KC后,继续培养12h,用RT-PCR和实时荧光定量RT-PCR法分别检测KC增殖和RARγ mRNA表达的改变。结果10-6mol/L依曲替酸或100mJ/cm2NB-UVB处理后12h,KC的增殖均受抑制,抑制率分别为8.7%和14.4%,二者联合作用时,对KC的增殖抑制作用最强,抑制率为19.7%。正常人KC可表达一定量的RARγ mRNA,10-6mol/L依曲替酸处理后,RARγ mRNA的表达增高至正常对照组的2.3倍,100mJ/cm2NB-UVB照射对RARγ mRNA的表达无明显影响,为正常对照组的0.9倍,二者联合作用时,对RARγ mRNA的表达诱导作用最强,为正常对照组的2.8倍。结论依曲替酸和NB-UVB可协同诱导RARγ mRNA表达,并可能参与介导二者对KC增殖的抑制作用。Objective To investigate the changes in cell proliferation and retinoic acid receptor γ（RARγ） mRNA expression in normal human keratinocytes after acitretin treatment and/or narrow-band ultraviolet-B irradiation. Methods Normal human keratinocytes were exposed to irradiation with 100 mJ/cm^2 NB-UVB and/or subsequent 12-hour incubation with 1×10^-6 mol/L acitretin, and the expression of RARγ/mRNA in the cells was examined using RT-PCR and real-time quantitative RT-PCR. Results A 0.9- and a 2.3-fold increase in RARγ/mRNA expression was induced in the cells by exposure to 100 mJ/cm^2 NB-UVB and 10^-6 mol/L acitretin, respectively, and the expression was synergistically enhanced by 2.8-fold after their combined treatment. Conclusion Upregulated expression of RARγ/ mRNA can be associated with keratinocyte growth inhibition after treatment with acitretin and NB-UVB irradiation.

关 键 词：角质形成细胞 窄谱中波紫外线 维甲酸受体 依曲替酸 

分 类 号：R96[医药卫生—药理学]