木犀草素对LPS诱导的RAW264.7细胞COX-2及mPGES-1表达的影响  

Effect of Luteolin on COX-2 and mPGES-1 Expression in LPS-induced RAW264.7 Cells

作　　者：王旭光[1] 陈根殷[1] 陈妙萍[1] 

机构地区：[1]广东医学院附属医院,广东湛江524100

出　　处：《中药材》2007年第10期1263-1266,共4页Journal of Chinese Medicinal Materials

基　　金：湛江市科技攻关项目(编号:2004090);广东医学院青年基金项目(编号:2004109)

摘　　要：目的:探讨木犀草素对脂多糖(LPS)诱导RAW264.7细胞COX-2及mPGES-1表达的影响。方法:酶免疫测定法(EIA)检测木犀草素对PGE2生成的影响,逆转录聚合酶链反应(RT-PCR)检测COX-2及mPGES-1 mRNA的表达。免疫印迹法(Western blotting)检测COX-2及mPGES-1蛋白的表达。结果:木犀草素抑制LPS诱导的RAW264.7细胞PGE2的生成,同时下调LPS诱导的RAW264.7细胞COX-2及mPGES-1 mRNA和蛋白的表达。结论:木犀草素可以抑制PGE合成途径中两个诱导酶COX-2和mPGES-1的表达。Objective： To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells. Methods： After being pretreated with different concentrations of luteolin for 30 min, and then incubated with 1μg/ml LPS for 12h, the effect of luteolin on the product of PGE2 in RAW264.7 cells was measured by enzyme immunoassay （EIA）, The mRNA expression of COX-2 and mPGES-1 in RAW264.7 ceils were analysed by RT-PCK The COX-2 and mPGES-1 protein expression in RAW264.7 ceils were analysed by western blotting, Results： Luteolin inhibited the LPS-induced PGE2 synthesis in RAW264. 7 cells, The mRNA and protein expression of COX-2 and mPGES-1 in RAW264.7 cells were also decreased by luteolin. Conclusion： Luteolin can inhibit significantly the expression of COX-2 and mPGES-1 in PGE2 synthetic pathway.

关 键 词：木犀草素 前列腺素E2 环氧合酶-2 膜相关前列腺素E2合成酶 

分 类 号：R285.5[医药卫生—中药学]