半枝莲提取物诱导人肺癌SPC-A-1细胞凋亡及对凋亡相关基因表达的影响  

Apoptosis-inducing Effect of Scutellaria barbata Extract on Human Lung Cancer SPC-A-1 Cells and the Expression of Apoptosis Associated Genes

作　　者：韦鹏涯[1] 浦洪琴[2] 韦星[3] 李朝敢[3] 农嵩[3] 

机构地区：[1]右江民族医学院微生物学教研室,广西百色533000 [2]右江民族医学院人体解剖学教研室,广西百色533000 [3]右江民族医学院生物化学与分子生物学教研室,广西百色533000

出　　处：《中药材》2007年第10期1270-1273,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨半枝莲提取物(Scutellaria barbataextract,SBE)对人肺癌SPC-A-1细胞凋亡及凋亡相关基因survivin和caspase-3表达的影响。方法:体外培养肺癌SPC-A-1细胞,以2.5、5、10 mg/L SBE处理SPC-A-1细胞48h,并以2.5 mg/L顺铂(DDP)作为阳性对照。用MTT法检测细胞抑制率,TUNEL法检测凋亡率,半定量RT-PCR检测survivin和caspase-3 mRNA表达。结果:与正常对照组相比,SBE各组细胞抑制率显著上升(P<0.001),细胞凋亡率明显升高(P<0.01),Caspase-3 mRNA表达显著上升(P<0.05或P<0.01),Survivin mRNA表达明显下降(P<0.05或P<0.01)。结论:SBE具有诱导肺癌SPC-A-1细胞凋亡的作用,其分子机制可能与上调caspase-3及下调survivin基因表达有关。Objective ： To explore the apoptosis-inducing effect of Scutellaria barbara extract （SBE） and the expression of apopto- sis associated genes survivin and caspase-3 on human lung cancer SPC-A-1 cells. Methods： Lung eancer SPC-A-1 cells were treated with 2. 5,5 and 10 mg/L for 48 h,and the cells were treated with 2.5 mg/L DDP as positive control. The inhibitory rat was evaluated by MTT assay. Apoptotic rate was determined by TUNEL method. The expression of survivin and caspase-3 mRNA were detected by semi-quantitive RT-PCR. Results ： Compared with control group, the inhibitory rate was increased obviously （ P 〈 0.001 ）, the apoptotic rate was increased markedly （ P 〈0. 01 ） , the expression of caspase-3 mRNA was increased significantly （ P 〈 0. 05 or P 〈 0. 01 ） , while survivin mRNA was decreased markedly （P 〈0. 05 or P 〈0. 01 ） in SBE groups. Conclusion： SBE can induce apoptosis of SPC- A-1 cells. The molecular mcchanism may be related to up-regulating expression of easpase-3 and down-regulating expression of survivin genes.

关 键 词：半枝莲 SPC-A-1细胞 细胞凋亡 生存素 Caspase-3 

分 类 号：R285.5[医药卫生—中药学]