CFDA—SE和Hoechst两种荧光标记技术在嗅鞘细胞移植修复脊髓损伤中的应用  

Comparison of CFDA-SE and Hoechst Immunofluorescence Labeling Techniques in the Application of Olfactory Ensheathing Cells Transplantation to Promote Injured Spinal Repair

作　　者：刘劲松[1] 李溪[1] 吴仕峰[1] 赵峻[1] 李彪[1] 龚跃昆[1] 

机构地区：[1]昆明医学院第一附属医院骨科,云南昆明650032

出　　处：《昆明医学院学报》2007年第5期1-4,16,共5页Journal of Kunming Medical College

摘　　要：目的将CFDA-SE和Hoechst两种常用的细胞荧光标记技术在嗅鞘细胞移植修复脊髓损伤中的应用进行比较,选出一种更适合的方法进行推广.方法取20只脊髓完全横断的SD大鼠,其中10只移植用CFDA—SE标记的嗅鞘细胞,另10只移植用Hoechst标记的嗅鞘细胞。结果两种标记方法都能成功标记上,但Hoechst对移植后组织的污染率较高,CFDA—SE染色清楚,对受区组织干扰较少.结论CFDA—SE相比Hoechst在嗅鞘细胞移植修复脊髓损伤中进行荧光标记更清楚、更准确,值得推广。Objective To Compare the effect of CFDA - SE and Hoechst fluorescence labeling techniques in the application of olfactory ensheathing cells transplantation to promote injured spinal cord repair. Methods OECs labeled with CFDA -SE were transplanted into completely transected zone of 10 S -D rats＇ spinal cord, while the other 10 completely transected spinal cord injured S - D rats were transplanted with the OECs labeled with Hoechst. Results Though both of OECs labeled respectively with CFDA - SE and Hoechst can be detected, but Hoechst may contaminated the recipient spinal cord, while the OECs labeled with CFDA - SE showed distinct fluorescence and little recipient tissure interference. Conclusion CFDA - SE immunofluorescence labeling techniques is preferable than Hoechst labeling techniques in the application of olfactory ensheathing cells transplantation to promote injured spinal cord repair.

关 键 词：羧基荧光素乙酰乙酸 双苯亚甲胺 嗅鞘细胞 脊髓损伤 荧光标记 

分 类 号：R336[医药卫生—人体生理学]