醛酮类物质染毒对小鼠肺脏抗氧化能力的影响  

The Impact of Aldehydes and Ketones Combustion on Anti-oxidative Capacities of Lungs in Mice

作　　者：徐兰琴[1] 陶涛[1] 邓穗德[2] 吕嘉春[1] 冉丕鑫[3] 

机构地区：[1]广州医学院化学教研室,广东广州510182 [2]广州医学院附属市一人民医院检验科,广东广州510182 [3]广州医学院附属第一医院呼吸疾病研究所,广东广州510182

出　　处：《广州医学院学报》2007年第3期6-8,共3页Academic Journal of Guangzhou Medical College

基　　金：国家"十五"科技攻关项目(2001BA703B03(A));广东省重大科技攻关项目(B30301)

摘　　要：目的:通过模拟生物燃料烟气中主要醛酮类物质染毒小鼠观察其对小鼠肺脏抗氧化能力的影响。方法:选用小鼠40只,随机分为2组:实验组和对照组,每组20只,雌雄各半。取甲醛(0.0663 mg)、乙醛(0.1181 mg)、苯甲醛(0.147 mg)、丙酮(0.609 mg)加水至1000 ml,置于超声波雾化器中向已放入20只小鼠的染毒柜喷雾,每天喷雾1次,每次5 h,连续13 d。对照组放于另一柜中,除喷雾蒸馏水外,其它同实验组。以化学分析方法检测小鼠外周血和肺组织中丙二醛(MDA)含量、超氧歧化酶(SOD)与谷胱甘肽过氧化物酶(GSH-Px)活力。结果:染毒小鼠肺脏MDA含量明显高于对照组[(17.88±2.08)nmol/mgprotvs(8.00±2.89)nmol/mgprot,P<0.001)];SOD与GSH-Px活力明显降低[(24.08±2.04)NU/mgprotvs(38.77±8.95)NU/mgprot;(28.75±4.82)U/mgprotvs(34.76±1.43)U/mgprot],P<0.001),染毒小鼠外周血中MDA含量变化不明显[(6.06±1.91)nmol/mgprotvs(5.62±2.54)nmol/mgprot,P>0.05],但SOD与GSH-Px活力明显低于对照组[(81.78±8.83)NU/mgprotvs(108.63±14.13)NU/mgprot;(50.94±3.20)U/mgprotvs(60.46±4.14)U/mgprot,P<0.001]。结论:模拟生物燃料烟气中主要醛酮类物质染毒小鼠可影响肺脏的抗氧化能力。Objective：To explore the effects of aldehydes and ketones in lung of mice so as to understand the relation between aldehydes and ketones in smoke from biomass combustion and airway diseases such as COPD. Methods：Forty male and female mice were randomly divided into two groups, the test group and the blank control group. Twenty mice in the treatment group were sprayed with a simulated mixture containing formaldehyde （0. 663 mg/m^3 ）, acetaldehyde（ 1. 181 mg/m^3 ） ,ketone （6.09 mg/m^3 ）and benzaldehyde （ 1.47 mg/m^3 ） for 13 days,one time per day,5 hours per time. The mice in blank control group were sprayed with distillated water in the same manner as the test group. The levels of MDA and SOD, as well as GSH-Px activity in peripheral blood and lung tissue were analyzed. Compared with controls, the studied group presented with unremarkable change in peripheral blood MDA [ （6.06 ± 1.91 ） nmol/mgprot vs （5.62 ± 2.54） nmol/mgprot ] but significantly lowered activities of SOD and GSH-Px [ （81.78 ± 8.83 ） NU/mgprot vs （ 108.63 ± 14.13 ） NU/mgprot and （ 50.94 ± 3.20） U/mgprot vs （60.46 ± 4.14） U/mgprot, P 〈0.001 ]. Results： Compared with controls, the concentration of MDA in mice lungs increased [ （ 17.88 ± 2.08 ） nmol/mgprot vs （ 8.00 ± 2.89） nmol/mgprot, P 〈 0. 001 ] , and the activity of SOD and GSH-Px significantly declined in the test group [ （24.08 ± 2.04 ） NU/mgprot vs （ 38.77 ± 8.95 ） NU/mgprot ; （ 28.75 ± 4.82 ） NU/mgprot vs （ 34.76 ± 1.43）U/mgprot,P 〈0. 001 ]. Conclusion： Inhalation of aldehydes and ketones in the same quantity as that of in sampling site of biomass combustion smoke induces oxidative damage in mice lung.

关 键 词：醛酮类物质 MDA SOD GSH—Px 肺脏 小鼠 

分 类 号：R994.3[医药卫生—毒理学]