荷斯坦乳牛乳腺和乳腺上皮细胞中Toll样受体及辅助因子编码基因的检测  

Detection of genes encoding Toll-like receptors and accessory molecules in mammary gland and mammary epithelial cells from Holstein cows

作　　者：王亨[1] 孟霞[2] 邱昌伟[1] 吴培福[1] 韩超[1] 齐长明[1] 

机构地区：[1]中国农业大学动物医学院临床兽医系 [2]中国农业大学生物学院微生物学与免疫学系,北京100094

出　　处：《中国兽医科学》2007年第10期882-886,共5页Chinese Veterinary Science

基　　金：科技部"十五奶业专项"(2002BA518A07)

摘　　要：参照牛TLR4、TLR2、CD14、MD-2基因序列设计了相应基因的引物。采用RT-PCR技术检测了体外培养的荷斯坦乳牛乳腺和乳腺上皮细胞中Toll样受体TLR4、TLR2及辅助因子CD14、MD-2基因。结果显示,乳腺上皮细胞中存在TLR4、TLR2、CD14和MD-2四个基因的表达,而乳腺中除MD-2未检测到外,其余3个基因均扩增成功。说明该受体及辅助因子可能参与了乳腺的先天性免疫防御。该研究为探讨乳腺的先天性免疫及乳腺上皮细胞在乳腺先天性免疫中的作用奠定了基础。Primers to amply TLR4,TLR2,CD14 and MD-2 genes were designed according to the related bovine sequences and were applied to detect the genes in mammary gland and mammary epithelial cells from Holstein cows cultured in vitro by RT-PCR. The four genes were expressed in the bovine mammary epithelial cells. With the exception of MD-2 gene, the remaining genes were detected in bovine mammary tissues. Innate immunity defense may be reinforced with the presence of the receptors and accessory molecules. These results provided a foundation for understanding the innate immunity of mammary gland and for further studies on the role of mammary epithelial cells in innate immunity of mammary gland.

关 键 词：牛 乳腺 TOLL样受体 辅助因子 先天性免疫 

分 类 号：S852.4[农业科学—基础兽医学] Q503[农业科学—兽医学]