猪NDUFS2基因的定位、克隆和组织表达谱分析  

Mapping,Cloning and Expression Profile Analysis of Porcine NDUFS2 Gene

作　　者：唐文花[1] 李勇[1] 王恒[1] 唐中林[2] 朱正茂[2] 杨述林[2] 靳二辉[1] 公维华[1] 李奎[2] 

机构地区：[1]华中农业大学动物科技学院,武汉430070 [2]中国农业科学院北京畜牧兽医研究所中国农业科学院家养动物遗传资源与种质创新重点开放实验室,北京100094

出　　处：《畜牧兽医学报》2007年第11期1137-1142,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30571300);国家"973"计划(2005DKA21101);"863"计划(2006110Z1039)

摘　　要：利用辐射杂种克隆板对猪NDUFS2(NADH dehydrogenase(ubiquinone)Fe-S protein 2)基因进行了染色体定位,克隆了该基因的CDS序列,用相关软件进行了基因结构和功能预测,并利用半定量RT-PCR对基因的组织表达谱进行了分析。研究结果表明:NDUFS2基因定位于猪的4号染色体SSC4q,与标记SW589紧密连锁。获得NDUFS2基因的CDS为1 392 nt,编码463个氨基酸。物种间系统进化树分析表明:猪与牛的NDUFS2基因序列同源性更高。结构预测发现猪NDUFS2基因具有一个NuoD保守结构域,与能量的产生和转化功能有关。根据组织表达谱分析结果,NDUFS2在猪的11种组织中的表达量有差异。In this study, we completed the chromosomal mapping of porcine NDUFS2 （NADH dehydrogenase （ubiquinone） Fe-S protein 2）gene using a radiation hybrid panel, cloned the coding sequence, predicted the structure and function of NDUFS2 gene with some software tools, and analyzed the expression profile of NDUFS2 gene in 11 different porcine tissues with semiquantitative RT PCR. The NDUFS2 gene was physically mapped on porcine chromosome 4q and closely linked to marker SW589. The coding sequence of porcine NDUFS2 gene is 1 392 nt, coding for 463 amino acids. The results of phylogenetic analysis among different species indicated that the porcine NDUFS2 gene is more homologous to bovine NDUFS2 gene. According to the structural prediction, porcine NDUFS2 gene has a conserved NuoD domain involved in generating and transforming of energy. The results of tissue expression profile showed that the expressive levels of NDUFS2 gene were different in 11 porcine tissues.

关 键 词：猪 NDUFS2基因 定位 克隆 表达谱 

分 类 号：S828.2[农业科学—畜牧学]