绵羊微卫星标记与生长性状的相关研究  

Correlation Analysis of Growth Traits in Native Sheep Using Microsatellite DNA Markers

作　　者：王建民[1] 岳文斌[1] 秦孜娟[2] 曲绪仙[3] 马月辉[4] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]山东农业大学动物科技学院,泰安271018 [3]山东省畜牧兽医总站,济南250022 [4]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《畜牧兽医学报》2007年第11期1160-1167,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省肉羊良种工程项目(SDLZ05RY0203);国家自然科学基金(30371045)

摘　　要：以相邻地理区域的4个地方绵羊品种133只个体为研究对象,选用位于绵羊第1-8号常染色体上的11个微卫星基因座,采用微卫星DNA技术和最小二乘线性模型,分析了与绵羊生长性状相关的标记效应。结果表明：正常发育情况下,不同生长性状之间相关极显著（P〈0.01）,除尻长外的生长性状与年龄相关不显著（P〉0.05）。品种因子对所有生长性状的效应（P〈0.000 1）大于年龄和标记因子。在8个基因座上发现与某些生长性状相关密切的标记效应（P〈0.05或P〈0.01）。影响相应性状的最有利基因型分别是BMS1248座位的136/136和BM3033座位的136/143与体高,BM6444座位的117/121与管围,BM3501座位的178/198与胸围,BMS710座位的118/130和BMS1724座位的178/168与体长,MAF70座位的142/144与体重,BM1227座位的108/116与尻宽。本研究还推测出上述基因座上对相应性状具有正效应或负效应的若干等位基因。Eleven polymorphic microsatellite loci in some were selected from GenBank. The relating were analyzed in 133 animals of four native sheep b sheep 1^st, 2^nd, 3^rd, 4^th, 6^th, 7^th and 8^th chromoeffects between genotypes and growth traits reeds from sympatric geography regions, by using least squares linear model and DNA analysis technique. The results indicated that,compared with age and marker factors, the breed factor had large effect on growth traits（P〈0. 000 1）. Eight microsatellite loci had significant effect on some growth traits of sheep（P〈0.05 or P〈0.01）. The most favourable genotypes for interrelated traits are 136/136 at BMS1248 and 136/ 143 at BM3033 for height at withers, 117/121 at BM6444 for circumference of cannon bone, 178/ 198 at BM3501 for heart girth, 118/130 at BMS710 and 178/168 at BMS1724 for body length, 142/144 at MAF70 for dody weight, 108/116 at BM1227 for thurl width, respestively. We also inferred that some alleles above loci had positive/ negative effect on interrelated growght traits.

关 键 词：地方绵羊品种 生长性状 微卫星基因座 遗传标记 

分 类 号：S826.2[农业科学—畜牧学]