利用线粒体D-loop区分析家鸭品种遗传多态性与系统进化  

Analysis on the Genetic Polymorphism and Systematic Evolution in Domestic Ducks by Mitochondrial DNA D-loop Region

作　　者：张汤杰[1] 李慧芳[2] 陈宽维[2] 常洪[3] 汤青萍[2] 张晶鑫[3] 

机构地区：[1]扬州大学兽医学院,扬州225009 [2]中国农业科学院家禽研究所,扬州225003 [3]扬州大学动物科学与技术学院,扬州225009

出　　处：《畜牧兽医学报》2007年第11期1168-1175,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30700572);江苏省高技术研究计划(BG2007323)

摘　　要：通过线粒体DNA控制区的结构和多态性来研究我国家鸭的遗传多态性与系统进化。利用DNA测序技术测定了我国9个家鸭品种106个个体线粒体DNA控制区多变序列。序列分析结果显示：A、C、T、G碱基的平均含量分别为25.6%、33.3%、15.2%和25.9%。检测到34个变异位点,约占分析位点总数的5.1%,有转换、颠换、插入/缺失4种类型的变异。确定了31种单倍型,其中单倍型A7为家鸭的主体单倍型,品种之间有9种共享单倍型。9个家鸭品种单倍型多样度（Hd）平均为0.798,核苷酸多样度（Pi）平均为0.28%,单倍型多样度在荆江麻鸭中最高,其次是攸县麻鸭和恩施麻鸭,在文登黑鸭中最低。9个品种家鸭之间双参数距离范围为0.001 3-0.004 4。31个家鸭单倍型序列的系统发生分析表明,9个家鸭品种只有1个母系起源,没有发现东亚斑嘴鸭对9个家鸭品种起源有贡献的证据。Investigation of the genetic polymorphism and systematic evolution in domestic ducks by the structure and polymorphism of mitochondrial DNA. The 667 bp control region on mitochondrial DNA from 106 domestic ducks of 9 breeds was sequenced. The result showed that content of nucleotide A,C,G,T were 25. 6%,33. 3%,15. 2% and 25.9%, respectively. There were 34 polymorphic sites （5.1% in total analyzed sites）. The transition, transversion, and insertion/ deletion were found in this region. 31 haplotypes were defined in this study, in which A7 was major haplotype in ducks and shared 9 haplotypes among breeds. The haplotype diversity（Hal） and average nucleotide diversity（Pi） were 0. 798 and 0.28%, respectively. Hd was the highest in Jingjiang Sheldrake, followed then by Youxian Sheldrake and Enshi Sheldrake, and lowest in Wendeng Black duck. Kimura 2-parameter distance between the breeds is 0. 001 3-0. 004 4. A Phylogenetic analysis of the 31 haplotypes identified that there was only one distinct maternal lineage in domestic duck breeds and revealed that no evidence of contribution of Anas zonorhyncha to the maternal origin of domestic duck breeds.

关 键 词：家鸭 MTDNA控制区 单倍型 遗传多样性 

分 类 号：S834.2[农业科学—畜牧学]