鸡贮精腺差异表达基因的研究  

Differentially Expressed Gene in Chicken Sperm Storage Tubules

作　　者：朱金金[1] 刘桂琼[1] 王志跃[1] 郭春燕[1] 黄军[1] 赖毓妍[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《畜牧兽医学报》2007年第11期1176-1179,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30300253)

摘　　要：用mRNA差异显示技术对鸡贮精腺中基因的差异表达进行研究,分离并筛选出1个差异表达片段SST01,经测序和BLAST分析,发现SST01与鸡2号染色体上的neurexophilin基因高度同源(98%)。Neurex-ophilin是一种只在神经细胞中分泌的糖蛋白,说明神经系统参与贮精腺内精子的贮存或释放。Neurexophilin作为精液素的配体发挥功能,而精液素使细胞粘连,从而说明neurexophilin参与精子在贮精腺内的贮存过程。The difference of gene expression in chicken sperm storage tubules （SST） was investigated by differential display reverse transcriptase polymerase chain reaction （DDRT-PCR）. One specific fragment, named SST01, was found. After secquencing and BLAST analysis, SST01 displayed high homology （98%） to neurexophilin gene of chicken. Neurexophilin is a glycoprotein which is detected only from neurons, which indicated that nervous system be related to the storage or release of sperm in SST. Neurexophilin functions as the ligand of neuridine which can cause cytoadherence, which indicated that neurexophilin be related to the storage of sperm in SST.

关 键 词：鸡 贮精腺 差异显示PCR neurexophilin 

分 类 号：S831.2[农业科学—畜牧学]