一株新城疫重组强毒的分离鉴定与分子特性分析  

Molecular Characterization and Identification of A Velogenic Newcastle Disease Virus Isolate

作　　者：谭雷涛[1] 秦卓明[1] 马保臣[2] 徐怀英[1] 崔治中[2] 

机构地区：[1]山东省农业科学院家禽研究所,济南250100 [2]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2007年第11期1217-1223,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省科技攻关重点支持项目(030317);山东省自然基金支持项目(031020101)

摘　　要：从患病蛋鸡中分离出1株新城疫病毒（NDV）SRZ/03,经蚀斑纯化后接种40日龄SPF鸡可诱发典型ND病变。蚀斑纯化前、后,MDT分别为55.2和51 h,ICPI为1.7和2.0,IVPI为1.91和2.79,表明属强毒。对其F和HN基因分别克隆测序,进行F基因分型。结果表明SRZ/03属基因Ⅱ型,F蛋白氨基酸裂解位点的序列为^112R-R-Q-K-R-F^117,与强毒基序完全相同。结合GenBank中发表的其他NDV参考株序列,对F和HN基因推导氨基酸序列进行比较。SRZ/03株与基因Ⅱ型LaSota/46、B1/47、Bingham/87和Texas/48的同源性分别为93.1%、93.0%、94.4%和94.4%;与基因Ⅶ型Taiwan/95、SGM/01和SKY/03的同源性分别为92.2%、92.8%和92.8%;与基因Ⅲ型F48E9/48的同源性为91.2%。然而,SRZ/03株F蛋白前210个氨基酸与基因Ⅱ型LaSota/46和Texas/48的同源性较高,达96.7%和98.1%,后343个氨基酸则与Taiwan/95、SGM/01等毒株的同源性较高,达95.7%-97.4%,推测该株为重组株。此外,SRZ/03株与Taiwan/95、SGM/01等毒株的HN氨基酸同源性高达95.8%-97.6%,显著高于与LaSota/46、B1/47、Bingham/87、Texas/48和F48E9/48的87.2%-89.2%。A Newcastle disease virus （NDV） field strain named SRZ/03 was isolated from a layer flock with typical symptoms and lesions and identified by the HI test, and cloned by plaque-purification of three times. SRZ/03 was determined as a virulent strain with MDT of 55.2h and 51h , ICPI of 1.7 and 2.0, IVPI of 1.91 and 2.79 respectively before and after plaque-purification. Analysis of F gene indicated that SRZ/03 belonged to genotype Ⅱ, and the amino acid sequence deduced from the F gene at the cleavage site was ^112R-R-Q-K-R-F^117 which was the same as the velogenic virus, and the F protein amino acid sequence had 93.1%, 93.0%, 94.4% and 94.4% homologies with that belong to published genotype Ⅱ vaccine strains LaSota/46, B1/47, virulent strain Bingham/87 and Texas/48, and very similar homologies of 92.2%, 92.8% , 92.8% or 91.2% were found when comparing with Taiwan/95, SGM/01, SKY/03 and F48E9/48 that belong to published genotypes Ⅶ and Ⅲ. However, the former 210 amino acids of F protein of SRZ/03 shared 96.7% and 98. 1% homology with that of LaSota/46 and Texas/48, respectively, and the highest homology of the latter 343 amino acids of F protein was 95.7% and 97.4% compared with Taiwan/95 and SGM/01, which indicated that SRZ/03 may be a recombinant strain.Furthermore, when HN genes were compared, SRZ/03 demonstrated higher homologies of 95.8%-97.6% with the known velogenic strains such as Taiwan/95 or SGM/01 , but lower homologies of 87.2%-89.3% with LaSota/46, B1/47, Bingham/87 and Texas/48.

关 键 词：新城疫病毒 毒力 F基因 HN基因 重组株 

分 类 号：S825.659.5[农业科学—畜牧学]